A Novel HPLC Method for Determining Three Effective Components in Chinese Medicine 'Xiao' Erjiebiao Granules

Abstract

A simple, rapid and reproducible HPLC method was used for the simultaneous determination of chlorogenic acid, baicalin and forsythin in Xiao'erjiebiao Granules, at the same time, response surface methodology (RSM) was devoted to forecast the best extraction technology. The HPLC System included an Agilent ZORBAX SB-C18 column ( 250 mm × 4.6 mm, 5μm ), acetonitrile (A) - 0.4% ethylic acid (B) as mobile phase with gradient elution at a flow rate of 1.0 mL.min-1 and detection wavelength of 327 nm for chlorogenic acid, 280 nm for baicalin and forsythin, respectively. The good linearity was achieved successfully with R2 <; 0.9995 in its standard curve aspects. Recoveries measured at the same concentrations (n = 6) were in the range of 96.4-97.6%. The established assay is accurate and practical, which could be utilized as a quality evaluation and control for Xiao'erjie biao Granules.