EVALUATION OF IMMUNE SYSTEM STIMULATION WITH VACCINE PREPARED AGAINST INDUCED BREAST CANCER IN ALBINO MICE

Ruaa Sh, S. Mohammed, Hamid Naji
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Abstract

This study was designed to prepare vaccines. Cancer vaccines promote the destruction of cancer cells,and the cancer cells contain special antigens on their surface when the vaccine is given, it acts as an antigen to activate the immune system. The IL-2 stimulates the response to a type of cells called T-cell, and outer membrane vesicle (OMV) targeting cancer cells by stimulating the immunity to respond with it two types including innate and adaptive immunity, that lead to stimulating the immune system to reduce mammary adenocarcinoma induction in lab mice using T4-1 cell line breast cancer by taking blood and serum to evaluate the immune system efficacy. The tumor induction success by monitoring mice body weight loss showed the lost weight began in the third week after tumor induction, so 23.2 g at first and second week to 14.35 g at the end of the fourth week, whereas the control animals were weighed 32.37 g. The immunity system efficacy results appear a difference in blood and serum parameters after cancer induction. The result shows an increase in total WBC and monocytes (5900, 0.2 cells/mm respectively) but non significantly decreased in neutrophils and lymphocytes count (2.6, 5.9 cells/mm, respectively). Therefore, the first and second doses of vaccines increased the antibody and complement of the immune system compared with control. While Eliaza data for cytokines profile referred to elevated IL2 (26.5 pg/ml)in the serum of vaccination mice but only significantly decreased in IL6 and IL-22 amount (20.4 and 19.6 pg/ml respectively) comparing with control.
白化小鼠诱导乳腺癌疫苗对免疫系统刺激的评价
本研究旨在制备疫苗。癌症疫苗促进癌细胞的破坏,当注射疫苗时,癌细胞表面含有特殊抗原,它作为抗原激活免疫系统。IL-2刺激机体对t细胞和外膜囊泡(OMV)细胞的反应,通过刺激机体对固有免疫和适应性免疫两种类型的免疫反应,从而刺激机体的免疫系统,从而减少T4-1细胞系乳腺癌实验小鼠的乳腺腺癌诱导,通过采集血液和血清来评估免疫系统的效果。通过监测小鼠的体重减轻,肿瘤诱导成功的小鼠体重减轻开始于肿瘤诱导后的第三周,从第一周和第二周的23.2 g到第四周结束时的14.35 g,而对照组动物的体重为32.37 g。肿瘤诱导后的免疫系统效能结果在血液和血清参数上出现差异。结果显示WBC和单核细胞总数增加(分别为5900和0.2个细胞/mm),但中性粒细胞和淋巴细胞计数无显著下降(分别为2.6和5.9个细胞/mm)。因此,与对照组相比,第一剂和第二剂疫苗增加了免疫系统的抗体和补体。而Eliaza细胞因子谱数据显示,与对照组相比,接种小鼠血清中il -2升高(26.5 pg/ml),但il - 6和IL-22的含量仅显著降低(分别为20.4和19.6 pg/ml)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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