The Effect of Goat Milk Yoghurt Casein Antioxidant Activity on KidneyTumor Necrosis Factor Alpha (TNF-α) and Interleukin 1 Beta (IL-1β) Levels in RattusnorvegicusExposed by 2,3,7,8-tetrachlorodibenzo-p- dioxin (TCDD)

Abstract

: Dioxins are highly toxic and carcinogenic compounds. The most dangerous dioxin is 2,3,7,8-tetrachloro-dibenzo-p-diozin (TCDD). TCDD can cause health problem accumulates overtime. Goat milk yoghurtcasein can reduce free radicals caused by TCDD. Goat milk yoghurt casein contains bioactive peptide that acts as antioxidant. The aim of the current research was to determine the effect of goat milk yoghurt towards TNF-α and IL1- β level after TCDD exposure. This research was an experimental study using a completely randomized design (CRD). Rattus norvegicus were divided into 6 groups: K- (negative control), K+ (positive control induced by TCDD 100 mg/kgBW), KP (placebo control given goat milk yoghurt casein 300 mg/kgBB), K1 (given TCDD 100 mg/kgBW and goat milk yoghurt casein 300 mg/kgBB), K2 (given TCDD 100 mg/kgBW and goat milk yoghurt casein 600 mg/kgBB), and K3 (given TCDD 100 mg/kgBW and goat milk yoghurt casein 900 mg/kgBB). Quantitative analysis withone-way ANOVA and post hoc Tukey test showed that goat milk yoghurt could prevent TNF- α increase significantly (p<0,05) from control groups, and also prevent IL1- β increase although not significantly different from control groups (p>0,05). It can be concluded that goat milk yoghurt casein 900 mg/kgBW can prevent the increase of TNF- α and IL1- β . Thus, goat milk yoghurt casein could be used as antioxidant source against TCDD exposure.