Assessment of Antioxidant, Antimicrobial, Cytotoxicity and Thrombolytic Potential of Erythrina fusca Lour Grown in Bangladesh

Bangladesh Pharmaceutical Journal Pub Date : 2022-01-31 DOI:10.3329/bpj.v25i1.57834

A. Anjum, Md. Zakir Sultan, Mohammad Abdullah Al Mansur, C. Hasan, M. Rashid

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引用次数: 3

Abstract

The current study was conducted to assess the antioxidant, antimicrobial, cytotoxicity and thrombolytic potential of the leaves and stem bark of Erythrina fusca Lour grown in Bangladesh. For the assessment of antioxidant properties, DPPH scavenging and phosphomolybdenum assays were used. In the DPPH free radical scavenging assay, the chloroform soluble fraction of a methanol extract of leaves of E. fusca exhibited the highest free radical scavenging activity with an IC50 value of 5.5 ± 0.60 μg/ml, while the reference standard (ascorbic acid) showed an IC50 value of 5.8 ± 0.18 μg/ml. All the test samples and the standard BHT demonstrated good linear relationship in the phosphomolybdenum assay. It was observed that the test samples contained considerable number of bioactive compounds including total phenolics in the aqueous soluble fraction of the leaves giving the highest 94.02 ± 1.004 GAE/100g of dried sample. All test samples exhibited moderate antimicrobial activity against 13 Gram-positive and Gram-negative bacterial strains and 3 fungi, where the chloroform soluble fraction of the stem bark demonstrated the highest inhibition of growth with zone of inhibition of 19.3 ± 0.21 mm against Bacillus cereus. All the test samples displayed cytotoxic activity against brine shrimp nauplii having significant LC50 and LC90 values. The chloroform soluble materials demonstrated highest lethality with LC50 value of 1.55 ± 0.30 μg/ml and LC90 65.68 ± 0.23 μg/ml. On the other hand, the carbon tetrachloride soluble fraction of the stem bark showed the highest clot lysis (22.75 ± 0.59%) as compared to that (66.77 ± 0.61%) revealed by reference standard, streptokinase. The findings of this study revealed that the leaves and stem bark of E. fusca growing in Bangladesh possesses bioactive compounds but further studies are required to purify and identify them. Bangladesh Pharmaceutical Journal 25(1): 1-8, 2022 (January)

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孟加拉国红藓的抗氧化、抗菌、细胞毒性和溶栓潜力评价

本研究旨在评估生长在孟加拉国的红藓(Erythrina fusca Lour)叶和茎皮的抗氧化、抗菌、细胞毒性和溶栓潜力。为了评估抗氧化性能，采用了DPPH清除和磷钼试验。在DPPH自由基清除实验中，云母叶甲醇提取物氯仿可溶性部位的IC50值最高，为5.5±0.60 μg/ml，而参比标准物(抗坏血酸)的IC50值为5.8±0.18 μg/ml。在磷钼测定中，所有样品与标准BHT均表现出良好的线性关系。结果表明，样品中含有相当数量的生物活性物质，其中叶水溶性部分的总酚类物质含量最高，为94.02±1.004 GAE/100g。所有样品对革兰氏阳性和革兰氏阴性菌株13株和真菌3株均表现出中等抑菌活性，其中茎皮氯仿可溶性部分对蜡样芽孢杆菌的抑菌效果最好，抑菌带为19.3±0.21 mm。所有样品均具有显著的LC50和LC90值，具有显著的细胞毒活性。氯仿可溶性物质的致死量最高，LC50为1.55±0.30 μg/ml, LC90为65.68±0.23 μg/ml。另一方面，茎皮的四氯化碳溶出率为22.75±0.59%，而参比标准品链激酶溶出率为66.77±0.61%。本研究结果表明，生长在孟加拉国的扶桑叶和茎皮具有生物活性化合物，但需要进一步的研究来纯化和鉴定它们。孟加拉国药学杂志25(1):1- 8,2022(1月)

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Bangladesh Pharmaceutical Journal

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