Effect of Escherichia Coli on Decorin and Type I Collagen Levels in Fetal Membranes of Premature Balb/c Mice

IF 0.2 Q4 PHARMACOLOGY & PHARMACY
Nirwan Nugraha, Suheni Ninik Hariyati, D. Gunawan, Mokhammad Nooryanto, Pande Made Dwijayasa
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Abstract

Introduction: Premature birth is a global problem in various countries worldwide. The pathogenesis leading to preterm labor may occur in early pregnancy and is associated with inflammatory pathways and changes in the angiogenic process. Decorin and biglycan are hypothesized to play a role in pregnancy maintenance, wherein preterm delivery, these levels are thought to be reduced. This study was examined effect of Escherichia coli on Decorin and Type I Collagen levels in fetal membranes of Premature Balb/c Mice Methods: There were 28 pregnant Balb/c mice divided into 4 groups, with a post-test-only control group design in true experimental design research. The first control group (N1) was a group of pregnant mice dissected on day-15, while the second control group (N2) were mice pregnant at term followed up to parturition. Group P1 were given Escherichia coli 1x109 CFU/ ml dose on the cervix on day-15, while the second treatment group (P2) were given Escherichia coli in the cervical canal on day-15 followed by delivery. We examined the Decorin and Type I Collagen levels between group. One-way ANOVA was used to analyze the collected data. Results: There was a significant difference in the mean decorin levels between the control group N1 vs P2 (12.35±2.24 ng/mL vs 6.62±1.50 ng/mL; p=0.000). In addition, a significant difference was also found in the mean level of type 1 collagen between the control group N1 vs P2 group (283.5±31.3 ng/mL vs 170.6±38.8 ng/mL; p=0.000). Conclusion: A decrease in decorin levels affects the reduction in collagen type 1 levels in fetal membranes of Balb/C mice in premature models.
大肠杆菌对早熟Balb/c小鼠胎膜装饰素和I型胶原蛋白水平的影响
前言:早产在世界各国都是一个全球性的问题。导致早产的发病机制可能发生在妊娠早期,与炎症途径和血管生成过程的改变有关。据推测,Decorin和biglycan在妊娠维持中发挥作用,其中早产,这些水平被认为是降低的。本研究旨在探讨大肠杆菌对早熟Balb/c小鼠胎膜中Decorin和I型胶原蛋白水平的影响。方法:将28只Balb/c妊娠小鼠分为4组,采用单纯试验后对照组设计,为真正的实验设计研究。第一对照组(N1)为第15天解剖的妊娠小鼠,第二对照组(N2)为足月妊娠随访至分娩的小鼠。P1组于第15天宫颈上给予大肠杆菌1x109 CFU/ ml剂量,第二治疗组(P2)于分娩后第15天宫颈管内给予大肠杆菌。检测各组间Decorin和I型胶原蛋白水平。采用单因素方差分析对收集的数据进行分析。结果:对照组N1组与P2组的平均decorin水平差异有统计学意义(12.35±2.24 ng/mL vs 6.62±1.50 ng/mL);p = 0.000)。此外,对照组N1组与P2组的1型胶原平均水平也有显著差异(283.5±31.3 ng/mL vs 170.6±38.8 ng/mL);p = 0.000)。结论:decorin水平的降低影响Balb/C小鼠胎膜1型胶原水平的降低。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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