H. Maeda, Kana Kobayashi, Toshifumi Watanabe, M. Satoh, F. Nomura, K. Sogawa
{"title":"Urinary carboxylesterase 5A fragment as an early diagnostic marker of cat chronic kidney disease","authors":"H. Maeda, Kana Kobayashi, Toshifumi Watanabe, M. Satoh, F. Nomura, K. Sogawa","doi":"10.2198/JELECTROPH.63.39","DOIUrl":null,"url":null,"abstract":"SUMMARY Chronic kidney disease (CKD) is a common disorder and cause of death in cats. In the classification proposed by the International Renal Interest Society (IRIS), stage I and II CKD are difficult to diagnose accurately using markers, in comparison with normal controls. We recently described a simple and highly reproducible two-step method for identifying potential disease-marker candidates among low-abundance urine proteins. Urine samples were taken from 56 normal control cats as the control group and from 56 cats with CKD (stage I). A carboxylesterase 5A fragment and filaggrin-2 fragment were identified as two proteins with higher levels in normal control cats. The performance of the ELISA of urine carboxylesterase 5A fragment was satisfactory in terms of recovery (97.2–102.4%) and within-run (1.3–3.6%) and between-day (1.5–4.1%) reproducibility. Urine carboxylesterase 5A fragment levels were significantly greater in normal cats (3.4±0.6 mg/dL) than in CKD (stage I) (1.9±0.5 mg/dL) (p<0.001). A carboxylesterase 5A fragment may be useful as a complementary marker to P-Cre and BUN for detection of CKD (stage I).","PeriodicalId":15059,"journal":{"name":"Journal of capillary electrophoresis","volume":"42 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of capillary electrophoresis","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2198/JELECTROPH.63.39","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
Abstract
SUMMARY Chronic kidney disease (CKD) is a common disorder and cause of death in cats. In the classification proposed by the International Renal Interest Society (IRIS), stage I and II CKD are difficult to diagnose accurately using markers, in comparison with normal controls. We recently described a simple and highly reproducible two-step method for identifying potential disease-marker candidates among low-abundance urine proteins. Urine samples were taken from 56 normal control cats as the control group and from 56 cats with CKD (stage I). A carboxylesterase 5A fragment and filaggrin-2 fragment were identified as two proteins with higher levels in normal control cats. The performance of the ELISA of urine carboxylesterase 5A fragment was satisfactory in terms of recovery (97.2–102.4%) and within-run (1.3–3.6%) and between-day (1.5–4.1%) reproducibility. Urine carboxylesterase 5A fragment levels were significantly greater in normal cats (3.4±0.6 mg/dL) than in CKD (stage I) (1.9±0.5 mg/dL) (p<0.001). A carboxylesterase 5A fragment may be useful as a complementary marker to P-Cre and BUN for detection of CKD (stage I).