Isomerization Dynamics of Wild Type and Mutated Anabaena Sensory Rhodopsin Mapped by Time-Resolved Coherent Raman Spectroscopy

2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) Pub Date : 2019-06-23 DOI:10.1109/CLEOE-EQEC.2019.8872608

P. Roy, Rei Abe-Yoshizumi, Yoshitaka Kato, H. Kandori, T. Buckup

{"title":"Isomerization Dynamics of Wild Type and Mutated Anabaena Sensory Rhodopsin Mapped by Time-Resolved Coherent Raman Spectroscopy","authors":"P. Roy, Rei Abe-Yoshizumi, Yoshitaka Kato, H. Kandori, T. Buckup","doi":"10.1109/CLEOE-EQEC.2019.8872608","DOIUrl":null,"url":null,"abstract":"The light induced isomerization of retinal protonated Schiff base (RPSB) inside the protein pocket is one of the fastest (<ps) and most stereo-selective photochemical reactions in nature [1]. The ground state structure of the RPSB and its surrounding protein architecture are known to play a central role in this reaction. It has been a longstanding question how each factor individually influences the reaction dynamics. In this context, Anabaena Sensory Rhodopsin (ASR), a recently discovered microbial retinal protein, serves as an ideal system to answer this question as it binds two structural isomers (all-trans and 13-cis) of the RPSB within the same protein constructions in its photocycle. In this work, the photo-isomerization dynamics of the RPSB in ASR has been explored with the help of time resolved coherent Raman techniques like pump-degenerate four-wave-mixing (pump-DFWM) (Fig. 1(a)) and pump-impulsive vibrational spectroscopy (pump-IVS). Both methods report on the structural changes of the RPSB during the photochemical reaction by tracking the transient frequency shifts of the vibrational modes in the excited state.","PeriodicalId":6714,"journal":{"name":"2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC)","volume":"57 1","pages":"1-1"},"PeriodicalIF":0.0000,"publicationDate":"2019-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/CLEOE-EQEC.2019.8872608","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}