Bioanalytical evaluation of dried plasma spot microsampling methodologies in pharmacokinetic studies applying Acetaminophen as model drug

Abstract

This paper reports the development and validation of different types of dried plasma spot bioassays and their application in a pharmacokinetic study. The pharmacokinetic study involved a single oral dose of the drug acetaminophen and post-dose blood samples were collected by finger prick. Plasma was separated for dried plasma spot preparation and thereafter quantitatively analyzed by liquid chromatography-accurate mass high resolution mass spectrometry. Comparison of results from bioanalytical performance experiments indicated that the two dried plasma spot bioassays worked well and observed acetaminophen pharmacokinetic values were found not to be statistically significantly different between the evaluated two dried plasma spot bioassays. More over, pharmacokinetic values observed were also not statistically significant different from values reported in literature. Overall, it was concluded that dried plasma spot bioassays could have a great potential as alternative sampling technique in pharmacokinetic studies.