Physicochemical Properties of four Phosphate-solubilizing Bacteria Isolated from the Semi-arid Regions of North Gujarat, India

Abstract

The rhizosphere of several plants was screened for phosphate solubilizing bacteria (PSB) from eight locations of North Gujrat. Thirty-three PSB were isolated in the study. Four PSB isolates with the highest phosphate solubilization index between 1.55–2 were characterized morphologically and biochemically and identified through 16S rRNAsequencing as Klebsiella aerogenes, Klebsiella pneumoniae, Kocuria flava and Enterobacter hormaechei. The growth of isolates was measured by plotting an optical density-based semi-logarithmic growth curve. The isolates were measured for Psolubilization in Pikovskaya broth. The isolate K. flava LC515414 -1solubilized maximum CaPO (7.63 μm P mL). The acid and alkaline phosphatase activity of 34the isolates was also measured for 96 h in the growth medium. After 96 h of growth, the isolate -1K. flava LC515414 had the highest final acid and alkaline phosphatase activity of 4.68 U Land -15.67 U L