Methylarsonic and dimethylarsinic acids toxicity and total arsenic accumulation in edible bush beans, Phaseolus vulgaris

Abstract

The main objective was to evaluate whether arsenic accumulated in the edible pods and seeds of Phaseolus vulgaris, cv. F15 above the Spanish maximum recommended concentration for food crops, 1 mg kg−1 on a fresh weight basis. Only organic arsenicals, methylarsonic and dimethylarsinic acids were used because they were: (1) the only arsenic species allowed for agricultural applications and (2) more mobile than inorganic species. Selection of French beans, a sensitive plant to arsenic, was based on the fact that arsenic-upward translocation is higher in sensitive than in tolerant plants. A 2 x 3 factorial experiment was conducted with two organic arsenic species (methylarsonic acid, dimethylarsinic acid) and three arsenic concentrations (0.2, 0.5, 1.0 mg l-1). Experimental results showed that the low bean plant tolerance to arsenic was possibly due to the high arsenic-upward transport to shoots, which could result in profound negative metabolic consequences. Even under extreme adverse conditions, arsenic residues in edible beans were below the maximum statutory limit set by the Spanish legislation. It is concluded that the major drawback of organic arsenical herbicides is that of decreased productivity rather than high arsenic intake by consumers of edible products from sensitive plant species.