Bioassay-Guided Isolation and Detection of a Cytotoxic Compound Known as Fucosterol from the Peruvian Lessonia Trabeculata Seaweed

Fern, R. ez, C. Areche, L. Quispe, X. Solis, B. Sepúlveda, Di, S. Zevallos, T. Cano
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Abstract

Lessonia trabeculata, called “Aracanto”, is a brown macroalga distributed in submarine, exposed and semiexposed rocky environments, and scattered along the Peruvian coast forming continuous grasslands. Despite its abundance in the Peruvian coastline, little or no importance has been given to its study, especially in the extraction of its secondary metabolites. In this work, we screened Lessonia trabeculata extracts for cytotoxic activity against human breast cancer cells. Only hexane extract was observed to be active against these cells. Both the chloroform and methanol extract were inactive. Bioassay-guided fractionation of the L. trabeculata hexane extract resulted in the isolation and identification of fucosterolusing NMR spectroscopy. The IC50 measured for hexane extract from L. trabeculata was 129 μg/mL and 75 μM for fucosterol, compared to mithramycin, a positive control. This study on the secondary metabolites of the Peruvian seaweed L. trabeculata is the first of its kind. The data obtained represents an interesting doorway for further studies.
从秘鲁小梁藻中分离和检测一种细胞毒性化合物focusterol
小梁藻(Lessonia trabeculata),又称“Aracanto”,是一种棕色大藻,分布在海底、暴露和半暴露的岩石环境中,散布在秘鲁海岸形成连续的草原。尽管它在秘鲁海岸线上丰富,但对它的研究很少或根本不重视,特别是在提取其次级代谢物方面。在这项工作中,我们筛选了小梁草提取物对人乳腺癌细胞的细胞毒活性。只有己烷提取物对这些细胞有活性。氯仿和甲醇提取物均无活性。生物测定法对小梁草己烷提取液进行分离,并利用核磁共振谱法对其进行鉴定。与阳性对照米霉素相比,小梁L.己烷提取物的IC50为129 μg/mL,焦甾醇的IC50为75 μM。本研究首次对秘鲁海藻L.小梁藻的次生代谢物进行了研究。获得的数据为进一步的研究提供了一个有趣的途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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