EFFICACY OF CHITOSAN AND CHITOSAN NANOPARTICLES TO CONTROL CHOCOLATE SPOT DISEASE ON FABA BEAN UNDER FIELD CONDITIONS

Abstract

This study was conducted at Plant Pathology Dep., Faculty of Agriculture, Damanhour University and Etay El-Baroud Agricultural Research Station, El El-Beheira governorate during 2018 - 2019 season. The aim of this study was to examine the efficacy of Chitosan and Chitosan nanoparticles to control chocolate spot disease caused by Botrytis fabae on faba bean (cv. Giza 40) under field conditions. Spraying Chitosan or Chitosan NP (30, 45, 60 after sowing) significantly decreased chocolate spot disease severity to 8.7% compared to 22.3 for the untreated control which was not significantly different from the fungicide Mancozeb which exhibited 10.17% disease severity. This was accompanied with an increase in totals phenols in Chitosan NP treated plants to 4.43 mg compared to 0. 436 for the untreated control. Also, this was accompanied with an increase in polyphenol and peroxidase activity to be as high as 0.399 and 0.394 compared to 0.133 and 0.130 (absorbance/min./g f.w.), in the untreated control, respectively. Meanwhile, use of Chitosan and Chitosan NP significantly improved faba bean vegetative growth, i.e . plant height, number of pods, as well as seeds yield per plant compared to the untreated control with more superiority for Chitosan NP.