Nitric oxide (NO) involved in Cd tolerance in NHX1 transgenic duckweed during Cd stress

IF 2.8 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Signaling & Behavior Pub Date : 2022-04-26 DOI:10.1080/15592324.2022.2065114

Qiuting Ren, Na Li, Ruxin Liu, Xueman Ma, Jinge Sun, Jianyao Zeng, Qingqing Li, Mingwei Wang, Xinglin Chen, Xiaoyu Wu, Lin Yang

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引用次数: 3

Abstract

ABSTRACT Anthropogenic activities cause heavy metal pollution, such as cadmium (Cd). Na+/H+ antiporter (NHX1) transgenic duckweed showed Cd tolerance in our previous study, and the signal mechanism needs to be explored. As an important signal molecule, nitric oxide (NO) is involved in a number of functions under abiotic stress response. This study analyzed the levels of endogenous NO in wild-type (WT) duckweed and NHX1 duckweed under Cd treatment. The results showed that after 24 h Cd treatment, the endogenous NO level of WT duckweed decreased, which was significantly lower than that in NHX1 duckweed. Studies have proved that NHX1 influences pH. The level of NO in this study has been investigated at different pH. The NO level was the highest in the duckweed cultured with pH 5.3. Nitrate reductase gene expression was down-regulated and NO synthesis was decreased under Cd stress in WT duckweed. This study showed that NO level has been modified in NHX1 duckweed, which could be influcened by pH.

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一氧化氮(NO)参与转NHX1基因浮萍在Cd胁迫下的Cd耐受

人为活动导致镉(Cd)等重金属污染。在我们前期的研究中，Na+/H+ antiporter (NHX1)转基因浮萍表现出Cd耐受性，其信号机制有待进一步探索。一氧化氮(NO)作为一种重要的信号分子，在非生物应激反应中参与多种功能。本研究分析了Cd处理下野生型(WT)浮萍和NHX1浮萍的内源NO水平。结果表明，Cd处理24 h后，WT浮萍内源NO水平降低，且显著低于NHX1浮萍。研究证明NHX1对pH有影响。本研究考察了不同pH下的NO水平，在pH为5.3时培养的浮萍中NO水平最高。Cd胁迫下，WT浮萍硝酸盐还原酶基因表达下调，一氧化氮合成减少。本研究表明，NHX1浮萍的NO水平受到pH的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Signaling & Behavior Agricultural and Biological Sciences-Plant Science

CiteScore

6.00

自引率

3.40%

发文量

111

期刊介绍： Plant Signaling & Behavior, a multidisciplinary peer-reviewed journal published monthly online, publishes original research articles and reviews covering the latest aspects of signal perception and transduction, integrative plant physiology, and information acquisition and processing.