Evaluation of Two Housekeeping Gene in Diagnosis of Helicobacter Pylori and Compare It to another Routine Test

Saja A. Al-Jumaili, Amina N. Althwani, Nawal M. Al-Khalidi
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Abstract

Helicobacter pylori consider as one of the major components of the stomach microbiome, this bacterium is prevalent worldwide and more than half of the world populations are infected with it. Various methods have been developed for the diagnosis and detection of infection with H. pylori by using molecular and routine techniques with different levels of specificity and sensitivity. to evaluate and compare two diagnostic methods for the detection of H. pylori bacteria in Iraqi patients, A total of 105 patients and 20 individuals as control with age ranged 17-85 years who attended the gastroenterology and hepatology tertiary center, Baghdad – Iraq, they diagnosed through physicians according to endoscopic findings. Furtherly Biopsy samples from different places were collected by gastroenterologists from each patient, used for histopathological and genetic detection of two Housekeeping gene (16SrRNA and amiA) by PCR technique. Peripheral blood (3 ml) was also withdrawn from patients, then each blood placed into gel tube for separate serum and measure the IgG antibody by ELISA technique. Result: Histological examination' show that 78(74.2%) were positive for H.pylori , while the detection of ' H. pylori by using molecular method has shown that 69 (65.7%) of the patients were positive for H. pylori by using 16SrRNA gene and only 47 (44.7%) where positive for amiA gene. By ELISA 92 (87.6%) of patients were positive for IgG antibody. Conclusion: There is no test that we can considered it as golden standard method for the diagnosis' of H.pylori, the combination of more than test is a good choice to achieve the most reliable result.
两个管家基因在幽门螺杆菌诊断中的价值及其与其他常规检查的比较
幽门螺杆菌被认为是胃微生物群的主要组成部分之一,这种细菌在世界范围内普遍存在,世界上一半以上的人口感染了它。各种方法已经开发用于诊断和检测感染幽门螺杆菌通过使用分子和常规技术具有不同水平的特异性和敏感性。为了评价和比较两种诊断方法对伊拉克患者幽门螺杆菌的检测,在伊拉克巴格达胃肠病学和肝脏病学三级中心就诊的105名患者和20名作为对照,年龄在17-85岁之间,他们通过医生根据内窥镜检查结果进行诊断。进一步由胃肠病学家从每位患者的不同部位采集活检标本,采用PCR技术对两个管家基因(16SrRNA和amiA)进行组织病理学和遗传学检测。取患者外周血3 ml,放入凝胶管分离血清,ELISA法测定IgG抗体。结果:组织学检查显示幽门螺杆菌阳性78例(74.2%),分子法幽门螺杆菌检测显示16SrRNA基因幽门螺杆菌阳性69例(65.7%),amiA基因幽门螺杆菌阳性47例(44.7%)。ELISA检测IgG抗体阳性92例(87.6%)。结论:没有一种检测方法可以作为诊断幽门螺杆菌的金标准方法,多种检测方法联合使用是获得最可靠结果的良好选择。
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