Evaluation of Two Housekeeping Gene in Diagnosis of Helicobacter Pylori and Compare It to another Routine Test

Abstract

Helicobacter pylori consider as one of the major components of the stomach microbiome, this bacterium is prevalent worldwide and more than half of the world populations are infected with it. Various methods have been developed for the diagnosis and detection of infection with H. pylori by using molecular and routine techniques with different levels of specificity and sensitivity. to evaluate and compare two diagnostic methods for the detection of H. pylori bacteria in Iraqi patients, A total of 105 patients and 20 individuals as control with age ranged 17-85 years who attended the gastroenterology and hepatology tertiary center, Baghdad – Iraq, they diagnosed through physicians according to endoscopic findings. Furtherly Biopsy samples from different places were collected by gastroenterologists from each patient, used for histopathological and genetic detection of two Housekeeping gene (16SrRNA and amiA) by PCR technique. Peripheral blood (3 ml) was also withdrawn from patients, then each blood placed into gel tube for separate serum and measure the IgG antibody by ELISA technique. Result: Histological examination' show that 78(74.2%) were positive for H.pylori , while the detection of ' H. pylori by using molecular method has shown that 69 (65.7%) of the patients were positive for H. pylori by using 16SrRNA gene and only 47 (44.7%) where positive for amiA gene. By ELISA 92 (87.6%) of patients were positive for IgG antibody. Conclusion: There is no test that we can considered it as golden standard method for the diagnosis' of H.pylori, the combination of more than test is a good choice to achieve the most reliable result.