Ophiostoma ulmi DNA naturally introgressed into an isolate of Ophiostoma novo-ulmi is clustered around pathogenicity and mating type loci

IF 0.3 4区农林科学 Q4 PLANT SCIENCES

Phytoprotection Pub Date : 2019-01-01 DOI:10.7202/1059303AR

Abdelali Et-Touil, M. Dusabenyagasani, G. Bouvet, C. Brasier, L. Bernier

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引用次数: 7

Abstract

The invasive fungal pathogens Ophiostoma ulmi and O. novo-ulmi have caused two successive pandemics of Dutch elm disease since the beginning of the 20th century. In nature, the highly aggressive O. novo-ulmi may hybridize with the less aggressive O. ulmi. Growth rate and molecular analyses were conducted on an unusual, moderately aggressive O. novo-ulmi isolate, AST27, carrying an introgressed pathogenicity gene, Pat1-m; on highly aggressive O. novo-ulmi isolate H327; on O. ulmi isolates Q412T and W9; and on progeny from laboratory crosses between H327 and AST27. Genetic analysis indicated that the Pat1 and Mat1 (mating type) loci were in different linkage groups corresponding to O. novo-ulmi H327 chromosomes 1 and 2, respectively. Most of the molecular differences between the nuclear genomes of H327 and AST27 occurred in the vicinity of Pat1 and Mat1. In addition, two putative quanti-tative trait loci, Mgr1 and Mgr2, which influence mycelial growth rate at 21°C and 28°C, the optima for O. novo-ulmi and O. ulmi, were linked to Mat1 and Pat1, respectively.

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自然渗入到新乌尔米蛇口的DNA聚集在致病性和交配型位点周围

自20世纪初以来，侵袭性真菌病原菌ulmi和O. novoo -ulmi引起了两次连续的荷兰榆树病大流行。在自然界中，高攻击性的O. novoo -ulmi可能与攻击性较弱的O. ulmi杂交。对一种罕见的中等侵袭性O. novoo -ulmi分离物AST27进行了生长速率和分子分析，该分离物携带渐渗致病性基因Pat1-m;对高侵袭性新芽孢杆菌分离株H327;菌株Q412T和W9的研究以及H327和AST27实验室杂交的后代。遗传分析表明，该位点分别位于O. novoo -ulmi H327染色体1号和2号对应的不同连锁群中。H327和AST27核基因组的分子差异大部分发生在Pat1和Mat1附近。此外，两个推测的数量性状位点Mgr1和Mgr2分别与Mat1和Pat1连锁，这两个位点在21°C和28°C条件下影响菌丝生长速率，这两个位点是O. novoo -ulmi和O. ulmi的最佳位点。

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Phytoprotection 生物-植物科学

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