Evaluation of α-Amylase Inhibitory, Antioxidant and Cytotoxic Activities of Mimusops elengi Seeds

Bangladesh Pharmaceutical Journal Pub Date : 2022-01-31 DOI:10.3329/bpj.v25i1.57836

A. Mamun, S. Easmin, A. Ahmed, Md. Rezaul Haque Ansary, Wasim Bary, Md Shahed Zaman, H. Haq

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引用次数: 1

Abstract

Mimusops elengi Linn., locally known as “Bakul, is a commonly used medicinal plant in Bangladesh to treat several maladities. The purpose of this study was to investigate the antioxidant, α-amylase inhibitory and cytotoxic activity of four different solvent fractions such as petroleum ether, chloroform, ethyl acetate, and water of M. elengi seed. In this study, the high ferric reducing (1.74 ± 0.005) and DPPH free radical scavenging activity (IC50 39.49 ± 0.05 μg/ml) was observed in the ethyl acetate fraction. The highest amount of flavonoid content (1059.80 ± 2.15 mg of CE/g) and total antioxidant capacity (0.17 ± 0.005) was present in chloroform fraction. On the other hand, the ethyl acetate fraction exhibited the highest total phenolic content (182.90 ± 0.38 mg of GAE/g). Crude methanolic extract showed highest inhibition of α-amylase with IC50 value of 4.09 ± 0.08 mg/ml. The brine shrimp lethality bioassay of ethyl acetate fraction exhibited maximum toxicity with low LD50 value 127.10 μg/ml. Thus, extracts of M. elengi seed could be a source of antioxidant, cytotoxic and α-amylase inhibitory activity containing compounds. Bangladesh Pharmaceutical Journal 25(1): 16-25, 2022 (January)

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蜜豆种子α-淀粉酶抑制、抗氧化及细胞毒活性评价

米索普·埃伦吉·林。它在当地被称为“巴库尔”，是孟加拉国常用的药用植物，可以治疗几种疾病。研究了石油醚、氯仿、乙酸乙酯和水等4种不同溶剂组分的抗氧化活性、α-淀粉酶抑制活性和细胞毒活性。在本研究中，乙酸乙酯部位具有较高的铁还原活性(1.74±0.005)和DPPH自由基清除活性(IC50为39.49±0.05 μg/ml)。黄酮类化合物含量最高(1059.80±2.15 mg CE/g)，总抗氧化能力最高(0.17±0.005)。乙酸乙酯部分总酚含量最高(182.90±0.38 mg GAE/g)。粗甲醇提取物对α-淀粉酶的抑制作用最强，IC50值为4.09±0.08 mg/ml。乙酸乙酯部分的盐水对虾毒力生物测定结果显示，其毒性最大，LD50值为127.10 μg/ml。因此，青霉种子提取物可能是含有抗氧化、细胞毒和α-淀粉酶抑制活性化合物的来源。孟加拉国药学杂志25(1):16- 25,2022(1月)

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Bangladesh Pharmaceutical Journal

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