Urea determination in FIA mode by a newly designed urease-based biosensor

Laboratory Robotics and Automation Pub Date : 1999-10-13 DOI:10.1002/(SICI)1098-2728(1999)11:5<266::AID-LRA4>3.0.CO;2-D

Stjepan Milardović, I. Kruhak, B. S. Grabarić

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引用次数: 7

Abstract

This article describes a new design of a urea biosensor developed by covalent immobilization of the enzyme urease on the top of a nickel-hexacyanoferrate-modified nickel electrode. The behavior of the electrode was described with respect to several experimental parameters in flow injection mode. Detection of urease-produced NH ion from parent urea at a working potential of 100 mV vs. a Ag/AgCl/2 M NaCl electrode showed linearity in the concentration range of urea in aqueous solutions from 10 μM to 10 mM. The influence of carrier electrolyte concentration, flow rate, and pH on the properties of the transducer were also examined. The selectivity recognition of the transducer was studied with respect to the influence of potentially interfering sodium and potassium ions. The electrode was tested over a period of 45 days. After this period, the biosensor lost only 20% of its sensitivity. © 1999 John Wiley & Sons, Inc. Lab Robotics and Automation 11: 266–271, 1999

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新设计的脲酶生物传感器在FIA模式下测定尿素

本文介绍了将脲酶共价固定在镍-六氰铁酸盐修饰的镍电极上的尿素生物传感器的新设计。在流动注射模式下，电极的行为与几个实验参数有关。在工作电位为100 mV时，采用Ag/AgCl/2 M NaCl电极，在10 μM ~ 10 mM的水溶液尿素浓度范围内，检测母本尿素产生的NH离子呈线性，并考察了载体电解质浓度、流速和pH对传感器性能的影响。在潜在干扰的钠离子和钾离子的影响下，研究了换能器的选择性识别。对电极进行了为期45天的测试。在此之后，生物传感器的灵敏度只下降了20%。©1999 John Wiley &儿子,Inc。机械工程学报，2009,31 (2):481 - 481

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Laboratory Robotics and Automation

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