Cytokine profile and oxidative stress parameters in women with initial manifestations of pelvic venous insufficiency

IF 0.4 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
M. Darenskaya, D. A. Stupin, A. Semendyaev, S. Kolesnikov, N. Semenova, L. Kolesnikova
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Abstract

Pelvic venous insufficiency (PVI) in women is widespread and is closely associated with the risk of reproductive disorders (in 15–25% of patients) and a high rate of the disease recurrence after treatment. The factors involved in venous wall damage include atherogenic stimuli and chronic endotoxin aggression due to inflammatory processes. The changes in the initial stages of the disease are usually minor and selective. There is currently an urgent need to identify initial markers of these changes to develop preventive measures for their correction. Therefore, the aim of this study was to determine the cytokine profile parameters' levels, as well as the activity of lipid peroxidation (LPO) and antioxidant defense (AOD) reactions in women with initial manifestations of PVI. Thirty-nine female patients with PVI (mean age 37.4 ± 9.1 years old) were the subjects of the study. The diagnosis was verified by clinical and instrumental examination including ultrasound angioscanning of the pelvic veins and therapeutic and diagnostic laparoscopy, and it was finally confirmed histologically. The control group included 30 nearly healthy women (mean age 33.5 ± 6.3 years old) who underwent surgical sterilization by laparoscopic access. Spectrophotometric, fluorometric and immunoassay methods were used in the study. The cytokine profile in female patients with PVI, as compared to the control group, was characterized by an increased concentration of proinflammatory (interleukin (IL) (IL-6) and IL-8) and anti-inflammatory cytokines (IL-4 and IL-10) and higher ratio values (IL-6/IL-10). The level of primary LPO products, conjugated dienes, was significantly increased and level of final products TBARs values was decreased in comparison to the control. The AOD system main enzyme activity, superoxide dismutase (SOD), was decreased, while the catalase activity increased. In patients with PVI, the glutathione reduced form concentration was lower than in the control group. The results of the study in women with PVI suggest negative changes in the cytokine profile and multidirectional changes in the indicators of the LPO system state in the initial stages of the disease. The control of these changes in patients with PVI should probably be an important component of preventive measures in the initial stages of the disease.
盆腔静脉功能不全女性初始表现的细胞因子谱和氧化应激参数
盆腔静脉功能不全(PVI)在女性中很普遍,与生殖障碍的风险密切相关(占患者的15-25%),治疗后疾病复发率高。静脉壁损伤的因素包括动脉粥样硬化刺激和炎症过程引起的慢性内毒素攻击。疾病初期的变化通常是轻微的和选择性的。目前迫切需要确定这些变化的初步标志,以便制定纠正这些变化的预防性措施。因此,本研究的目的是确定PVI初始表现女性的细胞因子谱参数水平,以及脂质过氧化(LPO)和抗氧化防御(AOD)反应的活性。39例女性PVI患者(平均年龄37.4±9.1岁)为研究对象。经盆腔静脉超声血管扫描及治疗诊断性腹腔镜等临床及仪器检查证实,最终病理证实。对照组为30例近健康妇女(平均年龄33.5±6.3岁),均行腹腔镜绝育手术。采用分光光度法、荧光法和免疫分析法。与对照组相比,女性PVI患者的细胞因子谱的特点是促炎(白细胞介素(IL) (IL-6)和IL-8)和抗炎细胞因子(IL-4和IL-10)的浓度增加,比值值(IL-6/IL-10)更高。与对照组相比,初级LPO产物共轭二烯水平显著增加,最终产物TBARs值水平降低。AOD系统主要酶超氧化物歧化酶(SOD)活性降低,过氧化氢酶活性升高。在PVI患者中,谷胱甘肽还原形浓度低于对照组。在PVI女性患者中的研究结果表明,在疾病的初始阶段,细胞因子谱出现负向变化,LPO系统状态指标出现多向变化。控制PVI患者的这些变化可能是疾病初期预防措施的重要组成部分。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
AIMS Medical Science
AIMS Medical Science MEDICINE, RESEARCH & EXPERIMENTAL-
自引率
14.30%
发文量
20
审稿时长
12 weeks
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