Study self-transmissibility of Salmonella Typhimurium plasmids virulence isolated from fresh chicken foods by conjugation

Evaluation Study of Three Diagnostic Methods for Helicobacter pylori Infection Pub Date : 2017-07-01 DOI:10.24271/garmian.167

S. Jabur, Aliaa A Hafz

{"title":"Study self-transmissibility of Salmonella Typhimurium plasmids virulence isolated from fresh chicken foods by conjugation","authors":"S. Jabur, Aliaa A Hafz","doi":"10.24271/garmian.167","DOIUrl":null,"url":null,"abstract":"The aim of the present investigation was experimentally the ability of the virulenceplasmid of Salmonella Typhimurium isolated from fresh chicken foods in al- Najaf Al-ashrafgovernorate to transfer between different isolates which might be the cause of variant diseasesin order to find new data about this objective that may help in study the control of infectionsin humans.S. typhimurium Lst1, Mst3 and Gst1 isolates ability to transfer t0 the transconjugant thenPCR results confirmed the frequency of spvA, pefA, rck and traT genes in E.colitransconjugant (TLst1) were (12%), (30%), (8%) and (26%) respectively where in (TMst1)transconjugant were(14%),(30%), (6%) and(28%) respectively, similar results appeared in(TGst1) transconjugant that were (6%),(17%), (4%)and (18%) respectively therefore presentstudy revealed according to conjugation experiments results that the virulence plasmid of S.typhimurium Lst1, Mst3 and Gst1 isolates were transmissible and perhaps lead to rapidincrease in virulence isolates among different bacterial species in Najaf governorate.","PeriodicalId":12283,"journal":{"name":"Evaluation Study of Three Diagnostic Methods for Helicobacter pylori Infection","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2017-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Evaluation Study of Three Diagnostic Methods for Helicobacter pylori Infection","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.24271/garmian.167","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

The aim of the present investigation was experimentally the ability of the virulenceplasmid of Salmonella Typhimurium isolated from fresh chicken foods in al- Najaf Al-ashrafgovernorate to transfer between different isolates which might be the cause of variant diseasesin order to find new data about this objective that may help in study the control of infectionsin humans.S. typhimurium Lst1, Mst3 and Gst1 isolates ability to transfer t0 the transconjugant thenPCR results confirmed the frequency of spvA, pefA, rck and traT genes in E.colitransconjugant (TLst1) were (12%), (30%), (8%) and (26%) respectively where in (TMst1)transconjugant were(14%),(30%), (6%) and(28%) respectively, similar results appeared in(TGst1) transconjugant that were (6%),(17%), (4%)and (18%) respectively therefore presentstudy revealed according to conjugation experiments results that the virulence plasmid of S.typhimurium Lst1, Mst3 and Gst1 isolates were transmissible and perhaps lead to rapidincrease in virulence isolates among different bacterial species in Najaf governorate.

查看原文本刊更多论文

用偶联法研究新鲜鸡肉中鼠伤寒沙门菌质粒毒力的自传性

本研究的目的是通过实验研究从al- Najaf al- ashrafal省新鲜鸡肉食品中分离的鼠伤寒沙门菌毒力质粒在可能引起变异疾病的不同分离株之间转移的能力，以期为研究人类感染的控制提供新的数据。pcr结果证实大肠杆菌转偶联菌(TLst1)中spvA、pefA、rck和traT基因的频率分别为(12%)、(30%)、(8%)和(26%)，而在(TMst1)转偶联菌中spvA、pefA、rck和traT基因的频率分别为(14%)、(30%)、(6%)和(28%)，在(TGst1)转偶联菌中也出现类似的结果，分别为(6%)、(17%)。(4%)和(18%)，因此本研究根据结合实验结果表明，鼠伤寒沙门氏菌Lst1、Mst3和Gst1分离株的毒力质粒具有传染性，可能导致纳贾夫省不同细菌种间毒力分离株的迅速增加。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Evaluation Study of Three Diagnostic Methods for Helicobacter pylori Infection

自引率

0.00%

发文量