Rapid analysis of the effect of aminoglycosides on bacteria by using a sensor system based on a piezoelectric resonator with a lateral electric field

Abstract

The possibility of express analysis of the effect of aminoglycoside antibiotics on bacteria (Escherichia coli) using a sensor system based on a piezoelectric resonator with a lateral electric field with an operating frequency range of 6–7 MHz is shown. E.coli strains, both sensitive and resistant to kanamycin were used for the experiments. During evaluating the kanamycin effect on bacteria, the change in the electrical impedance modulus of the resonator was used as an analitical signal. It has been established that the criterion for the antibiotic bacteria sensitivity is the change in the modulus of the sensor electrical impedance after antibiotic exposure on bacteria at any frequency near the resonance. The sensor is highly sensitive and allows diagnosing the antimicrobial susceptibility of bacteria within 7–9 minutes.