Tissue reconstruction of skin failures and soft-tissue injuries using regenerative medicine methods

Abstract

The electrospinning technique has been used to manufacture a composite material based on nanofibers made of aliphatic copolyamide and composite nanofibers made of chitosan and chitin nanofibrils. Experimental in vivo studies of the designed material as wound covering were carried out to treat a vast and multilayered wound on a rat's back. After 28 days and nights of observation, complete epithelialization of the wound surface was established to occur in the experimental rat group. Histological analysis of scar tissue showed the presence of a small minority of capillaries and a low amount of infiltrating cells. The survival of animals was 100%. At the same time, in the control group of animals, lethality was observed in 11% of cases, and suppurative complications were observed in 100% of cases. Thrombocyte gel prepared from the peripheral blood of the patients was used to increase the rate of tissue regeneration, and to reduce the infection probability. The curative effect was proved to increase when wound dressings and autologous blood product separation, i.e., thrombocyte gel and platelet-rich plasma, were applied simultaneously.