Role of NOS3 894G>T Gene Polymorphism in Traumatic Cerebral Hemorrhagic Contusion Khartoum, Sudan, 2020-2021

Abstract

The nitric oxide produced by the endothelial nitric synthase (NOS3) gene helps to maintain cerebral blood flow (CBF) after traumatic brain injuries (TBI). The aim is to determine the prognostic role of NOS3 894 G>T gene in traumatic hemorrhagic contusion and outcome. A cross-sectional study was conducted for 90 patients who attended the National Centre for Neurological Sciences, Khartoum, Sudan. Non-Sudanese patients, hemorrhagic contusions associated with other types of brain bleeding, and patients with chronic disease were excluded. An initial CT scan was used upon admission to detect brain edema, anatomical site, and the number of contusions. The Glasgow coma scale (GCS) was used upon admission to assess the trauma severity. The Glasgow outcomes scale (GOS) was used upon discharge to assess the outcome. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used for NOS3 genotyping. The result shows that 93.3% of patients were male, while 32.2% of them were female. It further indicates that 58.9 % had a mild injury; 60% of the patients were presented with frontal lobe injury, 14.4% had multiple sites injury, and 22.2% had brain edema. The number of deaths was 8 (8.9%). The genotyping of NOS3 revealed that 94.4% of patients had homozygous GG and 5.6% heterozygous GT. G allele represented 97.2% of NOS3 alleles. NOS3 894 G>T genotypes were not significantly associated with patients, linguistic afflation’ and outcome. The study concluded that NOS3 894G>T gene has no prognostic role in traumatic hemorrhagic contusion and outcome. Keywords: Nitric oxide synthase gene, Traumatic brain injury, Glasgow coma scale, Glasgow outcomes scale.