ANTIBACTERIAL EFFECTIVENESS OF Stenochlaena palustris LEAVES EXTRACT AGAINST THE GROWTH OF Streptococcus mutans

Dentino Jurnal Kedokteran Gigi Pub Date : 2022-10-28 DOI:10.20527/dentino.v7i2.14626

Mery Novita, I. W. A. Krishnawan Firdaus, Irham Taufiqurrahman

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引用次数: 1

Abstract

Background: Dental caries is a common chronic infectious disease of dental hard tissue resulting from the interaction between tooth structure, the microbial biofilm formed on the tooth surface, dietary as well as salivary influences. Streptococcus mutans are considered to be the main bacteria that play role in the formation of cariogenic biofilms. Chlorhexidine gluconate 0,2% is a commonly used mouthwash and considered as the gold standard. The long-term use of chlorhexidine gluconate 0,2% may cause side effects so an alternative mouthwash with natural ingredients is needed. Kelakai leaves extract contains bioactive compounds which has antimicrobial effects such as flavonoids, alkaloids, tannins and saponins which can be used as an alternative mouthwash for chlorhexidine gluconate 0.2%. Objective: The purpose of this study was to observe the antibacterial effectiveness of kelakai leaves extract with concentrations of 3.125%, 6.25%, 12.5%, 25%, 50%, 75% and 100% with chlorhexidine gluconate 0.2% on growth of Streptococcus mutans bacteria. Methods: This study conducted through true experimental laboratories with a post-test only research design with control group design. This study used 9 treatments with 5 repetitions so that there were a total of 45 samples. The treatment group was kelakai leaves extract with concentrations of 3.125%, 6.25%, 12.5%, 25%, 50%, 75% and 100% with two control groups namely chlorhexidine gluconate 0.2% and distilled water against Streptococcus mutans. Results: The MIC of kelakai leaves extract was set at a concentration of 12.5% and the MBC at a concentration of 50%. Conclusion: Kelakai leaves extract concentrations 50%, 75% and 100% has antibacterial effectiveness which equivalent to chlorhexidine gluconate 0,2% against Streptococcus mutans bacteria.Keywords: Antibacterial, Stenochlaena palustris leaves extract, Streptococcus mutans

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窄藻叶提取物对变形链球菌生长的抑菌作用

背景:龋齿是一种常见的牙齿硬组织慢性感染性疾病，是牙齿结构、牙表面形成的微生物生物膜、饮食及唾液等因素共同作用的结果。变形链球菌被认为是在龋齿生物膜形成中起作用的主要细菌。葡萄糖酸氯己定0.2%是常用的漱口水，被认为是黄金标准。长期使用0.2%的葡萄糖酸氯己定可能会产生副作用，因此需要一种含有天然成分的替代漱口水。克拉凯叶提取物含有具有抗菌作用的生物活性化合物，如黄酮类化合物、生物碱、单宁和皂苷，可作为0.2%葡萄糖酸氯己定的替代漱口水。目的:观察以葡萄糖酸氯己定为浓度分别为3.125%、6.25%、12.5%、25%、50%、75%和100%的克拉凯叶提取物对变形链球菌生长的抑菌效果。方法:本研究采用纯后测研究设计和对照组设计，在真实实验室内进行。本研究采用9个处理，5个重复，共45个样本。处理组为浓度为3.125%、6.25%、12.5%、25%、50%、75%和100%的克拉凯叶提取物，对照组为葡萄糖酸氯己定0.2%和蒸馏水。结果:克拉凯叶提取物的MIC浓度为12.5%，MBC浓度为50%。结论:克拉凯叶提取物浓度分别为50%、75%和100%，其对变形链球菌的抑菌效果相当于葡萄糖酸氯己定0.2%的抑菌效果。关键词:抗菌，窄藻叶提取物，变形链球菌

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Dentino Jurnal Kedokteran Gigi

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审稿时长

24 weeks