Factors affecting the efficiency of two-phase extraction of flavonoids and carotenoids from Hypericum maculatum L.

Abstract

Current problem in pharmacy is the creation of complex drugs based on Hypericum maculatum L., which have anti-inflammatory, astringent, antispasmodic and tonic effects. Hypericum herb contains a complex of biologically active substances of different polarity: flavonoids, hypericin, tannins, vitamins, carotenoids. Biphasic extraction solvents system allows, by one cycle, to simultaneously obtain two extracts, alcohol and oil, containing a complex of hydrophilic and lipophilic compounds. The dependence of the ratio of the polar and non-polar phases on the biologically active substances extraction from the Hypericum herb was studied. The effectiveness of two-phase extraction was determined by the content of flavonoids in the water-alcohol phases and carotenoids in oil phases. It was determined that a two-phase solvent system extracts a complex of biologically active substances of Hypericum maculatum L. more effectively than a sequential extraction with separate solvents of different polarities. The polar phase enhances the processes of desorption, diffusion and phase transfer of not only hydrophilic, but also lipophilic Hypericum herb substances. The optimal ratio of raw materials: 70% ethanol: oil, which provided the maximum yield of flavonoids and carotenoids from raw materials, was 1:10:10.