Efektivitas Biolarvasida Serai Wangi (Cymbopogon nardus) Dengan Daun Cengkeh (Syzygium aromaticum L.) Terhadap Larva Aedes aegypti

JURNAL ILMIAH SAINS Pub Date : 2021-09-22 DOI:10.35799/jis.v21i2.35616

C. L. Salaki, H. Wungouw, H. V. Makal

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引用次数: 2

Abstract

Penelitian ini menguji efektivitas kombinasi biolarvasida minyak atsiri serai wangi (Cymbopogon nardus) dengan daun cengkeh (Syzygium aromaticum l.) Terhadap larva nyamuk Ae. aegypti. Penelitian ini bertujuan untuk: (a) menganalisis pengaruh pemberian kombinasi biolarvasida minyak atsiri serai wangi dan daun cengkeh terhadap perkembangan larva Ae. aegypti, (b) menganalisis uji daya bunuh kombinasi biolarvasida serai wangi (sw) dan daun cengkeh (dc) terhadap larva Ae. aegypti, (c) menganalisis uji patogenisitas dari kombinasi biolarvasida serai wangi dan daun cengkeh terhadap larva Ae. aegypti. Uji kombinasi biolarvasida ini dilakukan terhadap larva nyamuk Ae. aegypti instar III dengan perlakuan variasi kombinasi konsentrasi “SW 2% (P)”, “ SW 0,75% ” + “DC1,25% (P1)”, “SW 1%” + “DC 1% (P2)”, “SW 1,25 %” + “DC 0,75% (P3)” , “DC 2% (P4)”. dan Kontrol air (P-). Themepos (P+). Parameter yang diamati meliputi gejala, persentase mortalitas dan waktu kematian. Mortalitas larva diamati pada jam ke-6,12,18,24 setelah aplikasi. Perbedaan proporsi mortalitas antar tingkat konsentrasi dengan control diuji dengan metode chi-kuadrat sedangkan lT50 dengan analisis probit. Hasil penelitian menunjukkan masing-masing kombinasi bahan uji (P,P1,P2,P3 dan P4) tidak memiliki perbedaan pengaruh yang sginifikan sebagai biolarvasida dengan taraf signifikansi sebesar 0,05.. Kesimpulan hasil penelitian ini bahwa kelima konsentrasi bahan uji tersebut tidak memberikan perbedaan pengaruh yang signifikan sebagai biolarvasida. Hal ini menunjukkan bahwa minyak atsiri serai wangi dan daun cengkeh baik diaplikasi secara tunggal maupun dikombinasi memiliki efektifitas larvasida yang sama dengan temephos dalam membunuh larva nyamuk Ae. aegypti. Waktu kematian (LT50) ekstrak serai wangi 12,72 jam, daun cengkeh 6,71 jam dan kombinasi 6,38 jam.Kata kunci: Biolarvasida; efektifitas; konsentrasi; patogenisitas The Effectiveness of Biolarvacide Essential Oil of Vitronella (Cymbopogon nardus) with Clove Leaves (Syzygium aromaticum.L.) on Ae. aegypti Mosquito Larvae ABSTRACTThis study tested the effectiveness of the biolarvicide combination of citronella essential oil (cymbopogon nardus) and clove leaf (syzygium aromaticum L.) against the larvae of Ae. aegypti. This study aims to: (a) analyze the effect of giving a combination of biolarvicides of citronella essential oil and clove leaves on the development of Ae. aegypti, (b) analyzing the killing power of the combination of citronella (sw) and clove leaf (dc) biolarvicides against Ae. aegypti, (c) analyzing the pathogenicity test of the combination of citronella and clove leaf biolarvicides against the larvae of Ae. Agypti. This biolarvicide combination test was carried out on the larvae of Ae. aegypti instar iii with treatment variations in the concentration combination “SW 2% (P)”, “SW 0.75%” + “DC1.25% (P1)”, “SW 1%” + “DC 1% (P2)”, “SW 1.25%” + “DC 0.75% (P3)” , “DC 2% (P4)”. and Water control (P-). Themepos (P+). Parameters observed included symptoms of mortality percentage and time of death. Larval mortality was observed at 6, 12, 18, 24 hours after application. The difference in the proportion of mortality between concentration levels and control was tested by the chi-square method while the lt50 method was tested by probit analysis. The results showed that each combination of test materials did not have a significant difference in effect as a biolarvicide with a significance level of 0.05. The conclusion of this study was that the five concentrations of the test material did not give a significant difference in effect as a biolarvicide. This indicates that the essential oils of citronella and clove leaves, either applied singly or in combination, have the same larvicidal effectiveness as temephos in killing the larvae of Ae. aegypti. Time of death (lt50) citronella extract 12.72 hours, clove leaf 6.71 hours and the combination 6.38 hours.Keywords: Biolarvicides; effectiveness; concentration; pathogenicity

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芳香生物拉西达(Cymbopogon nardus)和丁香叶(Syzygium芳香L)的效力。埃及的幼虫

这项研究测试了香精油(Cymbopogon nardus)和丁香叶(Syzygium芳香l)的生物溶剂组合的有效性。蚊子幼虫。埃及。本研究的目标是:(a)分析香料atsiri果胶油和丁香叶对Ae幼虫发育的影响。埃及，(b)分析香味草本植物和直属丁香叶的联合杀菌剂试验。埃及，(c)分析香料甘草杀虫剂和丁香叶对Ae幼虫的病原试验。埃及。对Ae蚊子幼虫进行联合杀虫剂测试。aegypti采用“SW 2% (P)”、“SW 0.75%”、“dc1.25%”、“SW 1%”(P1)、“DC 1.25%”(P2)、“DC 0.75%”(P3)、“DC 2% (P4)”的组合变化。和水控制(P-)。Themepos (P +)。观察到的参数包括症状、死亡率和死亡时间。幼虫死亡时间为6.12.18.24小时后观察。控制的浓度差异是用程分析法测试的，而lT50是用probit分析的。研究表明，不同的测试材料组合(P、P1、P2、P3和P4)在具有0.05显著意义的程度上，其作用与合成的生物杀虫剂没有显著差异。这项研究得出的结论是，这五种试验材料的浓度与生物杀虫剂没有显著影响。这表明，挥发油和多孔丁香叶在单独和组合中都能有效地使用同样的杀虫剂，杀死Ae蚊子的幼虫。埃及。死亡时间(LT50)香精油提精12.72小时，丁香叶6.71小时，共6.38小时。关键词:生物杀虫剂;有效性;浓度;生物降解的化学成分和苜蓿叶的病原性。埃及蚊子Larvae abstractti这一研究研究了citronella esmbopogon nardus oil和三叶草syzygium L对Ae Larvae的影响。埃及。这项研究说明:(a)分析提供柑橘油和丁香的生物降解效果，让Ae的发展继续下去。埃及，(b)分析西罗内拉和叶的结合力量反对Ae。埃及，(c)分析香橼和丁香对Ae幼虫的组合试验。Agypti。这种生物危害测试显示爱的智慧。集中治疗变量的aegypti，“SW 2% (P)”，“SW 0.75%”+“DC1”(P1)，“SW 1%”(P2)，“SW 1.25%”(P2)，“SW 1.25%”(P3)，“DC 0.75%”，“DC 2% (P4)”。水控制。Themepos (P +)。观察人员包括死亡时间和时间的死亡交响曲。应用程序后6、12、18、24小时观察死亡率。同步和控制之间平衡平衡的差异被chi平方的方法检测，而lt50的方法被probit analysis测试。结果表明，每一项材料试验的结果中，没有一种有效的不同，其影响是生物反应器，其浓度为0.05。这项研究的结论是材料测试的五种集中并没有考虑到生物反应器效应的严重差异。无论是用单独的还是用商业的形式使用的，都与杀死爱的果实的效果相同。埃及。死亡时间12小时72小时，丁香6.71小时和融合6.38小时安装:Biolarvicides;显示其;双臀;pathogenicity

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