Intestinal Parasitosis among Human Immunodeficiency Virus and Tuberculosis Infected Patients from Dharan, Nepal

Arjun Ghimire, Kishor Rai, H. Khanal
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Abstract

 Intestinal parasitic infestation reflects a health threat with patients living with HIV & Tu-berculosis (TB). Parasitic infections caused by protozoa and helminths are the most com-mon infections worldwide. The present study was done to find out the frequency of intes-tinal parasitosis among HIV and Tuberculosis (TB) patients of Dharan total of 53 samples (>15 years) from HIV seropositive patients and 28 samples (>20years) from TB patients were collected in a clean, dry and capped fitted container and subjected to macroscopic and microscopic examination for ova, cyst, adult parasites and or segments of parasites. Stool samples were fixed in a 10% formalin-ether solution. Sedimentation technique, with modified acid-fast (Ziehl-Neelsen) staining method, was performed for opportunistic in-testinal parasites in both patients. Multi-parasitic infection was noted in the study. The overall prevalence of intestinal parasites was found to be 54 (66.67%). This result had shown that the participation of male patients was comparatively higher than female pa-tients. Out of 81 stool samples, 15 (27.78%) G. lamblia, 12(22.22%) E. histolytica, 12 (22.22%) Cryptosporidium parvum, 6 (11.11%), Isospora belli 4 (7.40%) Microspridium, 1(1.85%) Blastocystis hominis, 2(3.70%) and Taenia spp, 2(3.70%). To prevent this infec-tion appropriate health education should be given to the patients concerning disease trans-mission, antiparasitic therapy, personal hygiene, and safe drinking water.
尼泊尔达兰地区人类免疫缺陷病毒和肺结核感染患者的肠道寄生虫病
肠道寄生虫感染反映了艾滋病毒和结核病(TB)患者的健康威胁。由原生动物和蠕虫引起的寄生虫感染是世界上最常见的感染。为了解达兰地区HIV和TB (TB)患者肠道寄生虫病的发生频率,收集HIV血清阳性患者53份(50 ~ 15年)和TB患者28份(50 ~ 20年)标本,置于干净、干燥、有盖的密闭容器中,进行虫卵、囊肿、成体寄生虫和(或)段寄生虫的宏观和显微镜检查。粪便样本固定在10%福尔马林醚溶液中。采用改良的抗酸(Ziehl-Neelsen)染色法沉淀法检测两例患者的机会性肠内寄生虫。研究中注意到多重寄生虫感染。肠道寄生虫总患病率为54(66.67%)。这一结果表明,男性患者的参与率相对高于女性患者。81份粪便标本中,兰螺旋体15例(27.78%),溶组织胞杆菌12例(22.22%),细小隐孢子虫12例(22.22%),细小隐孢子虫6例(11.11%),贝利等孢子虫4例(7.40%),人芽囊虫1例(1.85%),人带绦虫2例(3.70%),带绦虫2例(3.70%)。为了预防这种感染,应对患者进行适当的健康教育,包括疾病传播、抗寄生虫治疗、个人卫生和安全饮用水。
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