Immunohistochemical localization of a H2S-generating enzyme and its potential molecular target in hepatopancreas of the freshwater crayfish Procambarus clarkii

IF 0.9 4区生物学 Q3 MARINE & FRESHWATER BIOLOGY

Marine and Freshwater Behaviour and Physiology Pub Date : 2019-05-04 DOI:10.1080/10236244.2019.1659103

K. Wei, Yue Wei, Chang-Khil Song

{"title":"Immunohistochemical localization of a H2S-generating enzyme and its potential molecular target in hepatopancreas of the freshwater crayfish Procambarus clarkii","authors":"K. Wei, Yue Wei, Chang-Khil Song","doi":"10.1080/10236244.2019.1659103","DOIUrl":null,"url":null,"abstract":"ABSTRACT There is increasing evidence showing that the gasotransmitter hydrogen sulfide (H2S) and NF-κB-mediated signaling may play an important role in regulating immune response. However, essentially nothing is known about them in crustaceans, especially the red swamp crayfish (Procambarus clarkii). In this work, we used immunohistochemical method to investigate, for the first time, the presence and distribution of a H2S biosynthetic enzyme (cystathionine β-synthase, CBS) and a member of NF-κB family (RelA/p65) in crayfish hepatopancreas. Results indicated that (1) CBS-like and NF-κB p65-like immunopositive cells coexisted in hepatopancreas; (2) NF-κB p65 was diffusely localized and highly expressed in the cytoplasm of R- and B-cells, but CBS was observed mainly in connective tissue, especially in sinusoids; (3) and normally, at mean optical density (MOD) level, their expression was about 0.0032 ± 0.0028 and 0.0072 ± 0.0039, and proportion (%) of positive cells was 14.37 ± 5.76 and 23.5 ± 14.3, respectively.","PeriodicalId":18210,"journal":{"name":"Marine and Freshwater Behaviour and Physiology","volume":"63 1","pages":"121 - 129"},"PeriodicalIF":0.9000,"publicationDate":"2019-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Marine and Freshwater Behaviour and Physiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/10236244.2019.1659103","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MARINE & FRESHWATER BIOLOGY","Score":null,"Total":0}

引用次数: 3

Abstract

ABSTRACT There is increasing evidence showing that the gasotransmitter hydrogen sulfide (H2S) and NF-κB-mediated signaling may play an important role in regulating immune response. However, essentially nothing is known about them in crustaceans, especially the red swamp crayfish (Procambarus clarkii). In this work, we used immunohistochemical method to investigate, for the first time, the presence and distribution of a H2S biosynthetic enzyme (cystathionine β-synthase, CBS) and a member of NF-κB family (RelA/p65) in crayfish hepatopancreas. Results indicated that (1) CBS-like and NF-κB p65-like immunopositive cells coexisted in hepatopancreas; (2) NF-κB p65 was diffusely localized and highly expressed in the cytoplasm of R- and B-cells, but CBS was observed mainly in connective tissue, especially in sinusoids; (3) and normally, at mean optical density (MOD) level, their expression was about 0.0032 ± 0.0028 and 0.0072 ± 0.0039, and proportion (%) of positive cells was 14.37 ± 5.76 and 23.5 ± 14.3, respectively.

查看原文本刊更多论文

淡水螯虾克氏原螯虾肝胰腺中h2s生成酶及其潜在分子靶点的免疫组织化学定位

越来越多的证据表明，气体递质硫化氢(H2S)和NF-κ b介导的信号传导可能在调节免疫应答中发挥重要作用。然而，基本上在甲壳类动物中对它们一无所知，尤其是红色沼泽小龙虾(克氏原螯虾)。本研究首次采用免疫组织化学方法研究了小龙虾肝胰腺中H2S生物合成酶(胱硫氨酸β-合成酶，CBS)和NF-κB家族成员(RelA/p65)的存在和分布。结果表明:(1)肝胰腺中存在cbs样和NF-κB p65样免疫阳性细胞;(2) NF-κ b p65在R细胞和b细胞的细胞质中弥漫性定位并高表达，但CBS主要在结缔组织中观察到，尤其是在窦状细胞中;(3)正常情况下，在平均光密度(MOD)水平下，其表达量分别为0.0032±0.0028和0.0072±0.0039，阳性细胞比例(%)分别为14.37±5.76和23.5±14.3。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Marine and Freshwater Behaviour and Physiology 生物-海洋与淡水生物学

CiteScore

2.10

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Marine and Freshwater Behaviour and Physiology is devoted to the publication of papers covering field and laboratory research into all aspects of the behaviour and physiology of all marine and freshwater animals within the contexts of ecology, evolution and conservation. As the living resources of the world’s oceans, rivers and lakes are attracting increasing attention as food sources for humans and for their role in global ecology, the journal will also publish the results of research in the areas of fisheries biology and technology where the behaviour and physiology described have clear links to the contexts mentioned above. The journal will accept for publication Research Articles, Reviews, Rapid Communications and Technical Notes (see Instructions for authors for details). In addition, Editorials, Opinions and Book Reviews (invited and suggested) will also occasionally be published. Suggestions to the Editor-In-Chief for Special Issues are encouraged and will be considered on an ad hoc basis. With the goal of supporting early career researchers, the journal particularly invites submissions from graduate students and post-doctoral researchers. In addition to recognising the time constraints and logistical limitations their research often faces, and their particular need for a prompt review process, accepted articles by such researchers will be given prominence within the journal (see Instructions for authors for details).