Effect of Oral Honey Consumption on Immune Function Following Ex-Vivo Lipopolysaccharide Stimulation in Healthy Humans

International Journal of Nutrition Sciences Pub Date : 2019-03-12 DOI:10.14302/ISSN.2379-7835.IJN-19-2639

Olivia M McCarthy, O. Moser, M. Eckstein, Rachel Deere, S. Bain, G. Dunseath, O. Bodger, R. Bracken

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Abstract

Nine healthy individuals with a mean ± SD BMI of 22.0 ± 0.7 kg/m² and age of 20 ± 0.2 years, participated in this single-blind randomised, crossover trial investigating the impact of ingesting two different honeys (1) Tropical Forest Honey (TFH) and (2) Manuka Honey; strength 12+ (MAN) on circulating levels of plasma interferon gamma following ex-vivo lipopolysaccharide (LPS) stimulation. Blood samples were prepared into duplicate aliquots of whole blood (800 μl) and 100 μg/l of LPS was added to samples to give a final volume of 1 ml. Levels of IFN-γ in plasma fractions were measured via commercially available sandwich ELISA and all comparisons were made with paired data using the Wilcoxon Signed Rank test taking a significance level of 5%. Whilst significant intra-and-interpersonal variation was observed, IFN-γ concentrations remained statistically unchanged 48 hours after the ingestion of either honey (p=0.15). Thus, in this instance the type of honey did not influence the IFN-γ response to plasma samples spiked with LPS.

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口服蜂蜜对健康人体外脂多糖刺激后免疫功能的影响

9名健康个体，平均±SD BMI为22.0±0.7 kg/m²，年龄为20±0.2岁，参与了这项单盲随机交叉试验，研究摄入两种不同蜂蜜(1)热带森林蜂蜜(TFH)和(2)麦卢卡蜂蜜的影响;强度12+ (MAN)对体外脂多糖(LPS)刺激后血浆干扰素γ循环水平的影响。血液样品制备成两份全血(800 μl)，加入100 μg/l的LPS，最终体积为1 ml。血浆组分中IFN-γ的水平通过市售夹心ELISA检测，所有比较采用配对数据，采用Wilcoxon sign Rank检验，显著性水平为5%。虽然观察到显著的内部和人际差异，但摄入任何一种蜂蜜48小时后，IFN-γ浓度在统计学上保持不变(p=0.15)。因此，在这种情况下，蜂蜜的类型不影响IFN-γ对血浆样品中加入LPS的反应。

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