Microbicidal activity of crude extracts from Sargassum wightii against Bacillus cereus

International Current Pharmaceutical Journal Pub Date : 2014-09-05 DOI:10.3329/ICPJ.V3I10.20338

Amritha Krishna Bhuvaneshwari Venugopal, V. Thirumalairaj, Geetha Durairaj, M. Vijayan, N. Lazar, Kavya Kurumboor Sathyan, Amrutha Chacko, Rincy Yesudas, Shasna Kadheeja, Ishara Kumari John David, S. J. Damascene

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引用次数: 9

Abstract

The present study was investigated to explore the antibacterial activity of four different solvent (petroleum ether, chloroform, acetone and ethanol) crude extracts of marine brown seaweed Sargassum wightti. Crude extracts were screened against human pathogen Bacillus cereus. The antibacterial efficiency was performed by agar well diffusion, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methods . The results revealed that the crude extract of petroleum ether showed prominent inhibiting activity against B. cereus and other crude extracts showed below detectable level. The highest microbicidal activity (zone of inhibition) 9.0 ± 0.32 mm was obtained at the concentration of 250 μg /ml and the lowest activity was 3 ± 0.20 mm at 31.25 μg /ml concentration. The MIC and MBC values were found to be 125 and 250 μg /ml respectively. Results of this study suggested that the compounds present in the crude extracts of petroleum ether showed high activity against B. cereus and further studies are required to purify the active principles. DOI: http://dx.doi.org/10.3329/icpj.v3i10.20338 International Current Pharmaceutical Journal, September 2014, 3(10): 326-327

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马尾草粗提物对蜡样芽孢杆菌的杀菌活性研究

研究了四种不同溶剂(石油醚、氯仿、丙酮和乙醇)对褐藻的抑菌活性。对粗提物进行了抗蜡样芽孢杆菌的筛选。采用琼脂孔扩散法、最低抑菌浓度法和最低杀菌浓度法测定其抑菌效果。结果表明，石油醚粗提物对蜡样芽孢杆菌的抑制作用显著，其他粗提物的抑制作用均低于检测水平。在浓度为250 μg /ml时，活性最高(抑制区)为9.0±0.32 mm;在浓度为31.25 μg /ml时，活性最低为3±0.20 mm。MIC和MBC分别为125和250 μg /ml。本研究结果表明，石油醚粗提物中所含化合物对蜡样芽孢杆菌具有较高的活性，其活性机理有待进一步研究纯化。DOI: http://dx.doi.org/10.3329/icpj.v3i10.20338国际现代医药杂志，2014年9月，3(10):326-327

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International Current Pharmaceutical Journal

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