Development of a magnetic solid phase extraction method for gemcitabine from rat plasma by using magnetic graphene oxide calix[6]arene composite and its application to pharmacokinetics

IF 2.3 4区工程技术 Q3 CHEMISTRY, MULTIDISCIPLINARY

Separation Science and Technology Pub Date : 2023-04-18 DOI:10.1080/01496395.2023.2201392

Babji Palakeeti, Namburi L A Amara Babu, J. Chinta

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引用次数: 0

Abstract

ABSTRACT A highly accurate and precise HPLC-UV method was developed for identification and quantification of gemcitabine anti-cancer drug in rat plasma. A new magnetic graphene oxide-based calixarene composite was developed and used as a solid phase for extraction of gemcitabine from rat plasma. Extraction efficiency has been studied by varying different experimental variables (eluent type, sorbent amount, extraction time and eluent volume etc.) and these were evaluated and optimized. Magnetic solid phase extraction (MSPE) conditions such as amount of solid phase, extraction solvents and their amounts, and adsorption and desorption times were optimized for getting better recoveries. Under the optimized conditions, linearity was evaluated with good correlation coefficient value R2 (0.9993). Limit of detection (2.0 ng/mL) and limit of quantification (13.0 ng/mL) was assessed using signal-to-noise ratio method. Intraday precision RSD values of gemcitabine (GEM) were found to be less than 4.6% and interday precision values are less than 6.8%. The results obtained during the robustness study were RSD values between 1.2 and 4.6%; these results indicate that the method has effective performance and reliability. The recovery percentages of gemcitabine at three QC level concentrations were obtained in the range from 97.6 to 100.2%.

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磁性氧化石墨烯杯[6]芳烃复合物从大鼠血浆中提取吉西他滨的磁固相萃取方法及其在药代动力学中的应用

建立了一种高准确度、高精密度的大鼠血浆中抗癌药物吉西他滨的HPLC-UV鉴别定量方法。研制了一种新型磁性氧化石墨烯基杯芳烃复合物，并将其作为固相从大鼠血浆中提取吉西他滨。通过不同的实验变量(洗脱液类型、吸附剂用量、萃取时间、洗脱液体积等)对萃取效率进行了研究，并对其进行了评价和优化。对固相用量、萃取溶剂及其用量、吸附和解吸次数等条件进行了优化，以获得较好的回收率。在优化条件下，线性关系良好，相关系数R2(0.9993)。采用信噪比法评估检测限(2.0 ng/mL)和定量限(13.0 ng/mL)。吉西他滨(GEM)的日内精密度RSD值小于4.6%，日内精密度小于6.8%。稳健性研究的结果为RSD值在1.2 ~ 4.6%之间;结果表明，该方法具有有效的性能和可靠性。在3种QC水平浓度下，吉西他滨的回收率在97.6 ~ 100.2%之间。

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来源期刊

Separation Science and Technology 工程技术-工程：化工

CiteScore

6.10

自引率

3.60%

发文量

131

审稿时长

5.7 months

期刊介绍： This international journal deals with fundamental and applied aspects of separation processes related to a number of fields. A wide range of topics are covered in the journal including adsorption, membranes, extraction, distillation, absorption, centrifugation, crystallization, precipitation, reactive separations, hybrid processes, continuous separations, carbon capture, flocculation and magnetic separations. The journal focuses on state of the art preparative separations and theoretical contributions to the field of separation science. Applications include environmental, energy, water, and biotechnology. The journal does not publish analytical separation papers unless they contain new fundamental contributions to the field of separation science.