LncRNA GHET1 and LncRNA ZXF2 as New Biomarkers in Oral Squamous Cell Carcinoma in Relation to Clinicopathological Variables

IF 0.4 Q4 ONCOLOGY

International Journal of Cancer Management Pub Date : 2023-04-05 DOI:10.5812/ijcm-121372

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引用次数: 1

Abstract

Background: The utilization of molecular techniques in detecting cancer has resulted in an improved prediction of outcomes. Oral squamous cell carcinoma (OSCC) is a prevalent illness that is frequently detected in its late stages. Therefore, finding molecular biomarkers that lead to the early detection of OSCC is of great importance. Objectives: This study aimed at evaluating the expression levels of long non-coding RNA (LncRNA) gastric carcinoma highly expressed transcript 1 (GHET1) and lncRNA ZXF2 in OSCC patients and their relationship with clinical pathology variables due to biomarker discovery and early diagnosis of OSCC. Methods: Tissue sampling was performed after selecting 30 OSCC patients and 30 healthy individuals from Emam-Khomeini Hospital, Tehran, Iran. Then, RNA extraction and cDNA synthesis were performed from these samples, using the relevant kits and their quantity and quality were measured, using nanodrop and agarose gel electrophoresis, respectively. For molecular biomarker identification and validation, real-time PCR (RT-PCR) was utilized to assess the expression of lncRNA GHET1 and lncRNA ZXF2. Data analysis was done, using GraphPad prism V.8 software. Results: The results showed that the expressions of both lncRNA GHET1 and lncRNA ZXF2 in OSCC tumor tissue increased compared to normal tissue (P < 0.0001). Receiver operating characteristic (ROC) analysis indicated that lncRNA GHET1 and lncRNA ZXF2 have the capability to be employed as biomarkers for OSCC detection. However, no significant relationship was observed between lncRNA GHET1 and lncRNA ZXF2 expressions with clinicopathological variables such as tumor stage and grade as well as patients' age. Conclusions: LncRNA GHET1 and lncRNA ZXF2 have the potential to be used as biomarkers in the early detection of OSCC and evaluating their expression in clinical settings are recommended. The use of these biomarkers in the early detection of OSCC can prevent the high mortality rate of OSCC patients. In the current study, the important role of the studied lncRNAs in OSCC diagnosis was shown. However, further studies are needed to confirm this.

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LncRNA GHET1和LncRNA ZXF2作为口腔鳞状细胞癌新生物标志物与临床病理变量的关系

背景:分子技术在癌症检测中的应用提高了对预后的预测。口腔鳞状细胞癌(OSCC)是一种常见病，经常在其晚期被发现。因此，寻找早期发现OSCC的分子生物标志物具有重要意义。目的:本研究旨在评价长链非编码RNA (LncRNA)胃癌高表达转录物1 (GHET1)和LncRNA ZXF2在OSCC患者中的表达水平及其与OSCC生物标志物发现和早期诊断的临床病理变量的关系。方法:选取伊朗德黑兰Emam-Khomeini医院OSCC患者30例和健康人群30例进行组织取样。然后利用相关试剂盒提取RNA和合成cDNA，分别采用纳米滴法和琼脂糖凝胶电泳法测定其数量和质量。为了鉴定和验证分子生物标志物，利用实时荧光定量PCR (RT-PCR)检测lncRNA GHET1和lncRNA ZXF2的表达。使用GraphPad prism V.8软件进行数据分析。结果:结果显示，lncRNA GHET1和lncRNA ZXF2在OSCC肿瘤组织中的表达均高于正常组织(P < 0.0001)。受试者工作特征(ROC)分析表明，lncRNA GHET1和lncRNA ZXF2具有作为OSCC检测生物标志物的能力。而lncRNA GHET1和lncRNA ZXF2的表达与肿瘤分期、分级等临床病理变量及患者年龄无明显关系。结论:LncRNA GHET1和LncRNA ZXF2有潜力作为OSCC早期检测的生物标志物，推荐在临床环境中评估其表达。在早期发现OSCC时使用这些生物标志物可以防止OSCC患者的高死亡率。本研究显示了所研究的lncrna在OSCC诊断中的重要作用。然而，需要进一步的研究来证实这一点。

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来源期刊

International Journal of Cancer Management ONCOLOGY-

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： International Journal of Cancer Management (IJCM) publishes peer-reviewed original studies and reviews on cancer etiology, epidemiology and risk factors, novel approach to cancer management including prevention, diagnosis, surgery, radiotherapy, medical oncology, and issues regarding cancer survivorship and palliative care. The scope spans the spectrum of cancer research from the laboratory to the clinic, with special emphasis on translational cancer research that bridge the laboratory and clinic. We also consider original case reports that expand clinical cancer knowledge and convey important best practice messages.