Bio-Electrocatalytic Reduction of Hydrogen Peroxide by Peroxidase from Guinea Grass (Panicum Maximum) Immobilized on Graphene and Graphene Oxide Screen-Printed Electrodes

Q4 Engineering

Ingenieria y Universidad Pub Date : 2022-07-12 DOI:10.11144/javeriana.iued26.brhp

J. Castillo, Paula Andrea Guarin-Guio, Ludy Ortiz

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引用次数: 0

Abstract

Objective: In this article a comparison was made between graphene (SPGE) and graphene oxide screen-printed electrodes (SPGOE) to study the bio-electrocatalytic reduction of hydrogen peroxide (H2O2) by guinea grass peroxidase (GGP). Methods and materials: GGP was immobilized onto SPGE and SPGOE by a drop-casting procedure. Electrochemical techniques were carried out to monitor the electrochemical behavior of GGP and the efficiency of electrocatalytic reduction of H2O2. Results and discussion: GGP adsorbed on both electrodes exhibited a couple of well-defined redox peaks at 120 mV/10.5 mV and 184 mV/59 mV for anodic and cathodic peaks, respectively. Linearity between scan rates root and oxidation and reduction peak currents for both electrodes suggest a surface-controlled process. The GGP-modified electrodes exhibited a good electrocatalytic activity to H2O2 reduction at a redox potential of -0.6 V and -0.5 V for SPEG and SPEGO, respectively. Conclusions: SPGE and SPGOE electrodes modified with GGP showed excellent analytical performance towards different concentrations of hydrogen peroxide. This is a preliminary step to developing a bio-analytical portable system based on GGP for the detection of H2O2 in real environmental samples.

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石墨烯和氧化石墨烯丝网印刷电极固定化豚草过氧化物酶生物电催化还原过氧化氢研究

目的:通过比较石墨烯(SPGE)和氧化石墨烯丝网印刷电极(SPGOE)，研究几内亚草过氧化物酶(GGP)对过氧化氢(H2O2)的生物电催化还原作用。方法和材料:采用滴铸法将GGP固定在SPGE和SPGOE上。采用电化学技术监测了GGP的电化学行为和电催化还原H2O2的效率。结果和讨论:吸附在两个电极上的GGP分别在120 mV/10.5 mV和184 mV/59 mV的阳极峰和阴极峰表现出一对明确的氧化还原峰。扫描速率与氧化和还原峰值电流之间的线性关系表明，这是一个表面控制的过程。在-0.6 V和-0.5 V的氧化还原电位下，ggp修饰电极对SPEG和SPEGO的H2O2还原表现出良好的电催化活性。结论:GGP修饰的SPGE和SPGOE电极对不同浓度的过氧化氢均有良好的分析性能。这是开发基于GGP的便携式生物分析系统用于检测真实环境样品中的H2O2的初步步骤。

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来源期刊

Ingenieria y Universidad Engineering-Engineering (all)

CiteScore

0.80

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0.00%

发文量

期刊介绍： Our journal''s main objective is to serve as a medium for the diffusion and divulgation of the articles and investigations in the engineering scientific and investigative fields. All the documents presented as result of an investigation will be received, as well as any review about engineering, this includes essays that might contribute to the academic and scientific discussion of any of the branches of engineering. Any contribution to the subject related to engineering development, ethics, values, or its relations with policies, culture, society and environmental fields are welcome. The publication frequency is semestral.