Identification of Foot and Mouth Disease (FMD) Virus From Recently Outbreak Crossbred Cattle In Rajbari District of Bangladesh

M. S. Islam, M. Akter, MZ Hassan, M. Rahman, E. Islam, M. Khan, A. Chakrabartty, M. Giasuddin
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引用次数: 4

Abstract

Foot and mouth disease (FMD) is a devastating viral disease and endemic in nature in Bangladesh that causes huge economical losses. The present research work was aimed to determine the prevalence of FMD outbreaks and molecular detection of FMDV serotypes by uRT-PCR and gsRT-PCR test, respectively from crossbred cattle in the Rajbari district of Bangladesh during the period from January to June 2018. A total of 16 tongue epithelial samples were collected from clinically FMD suspected 2 to 3 years old crossbred cattle. 14 samples were positive by uRT-PCR. The detection rate of FMDV by uRT-PCR was 87.50%. Then uRT-PCR positive samples were serotype by gsRT-PCR. Serotype based prevalence of FMDV was 42.86%, 100%, 21.43% and 21.43% in O serotype, Asia-1 serotype, A serotype and mixed infection with Asia-1 and A, respectively. Considering the age, the prevalence of confirmed FMD outbreak was 42.86%, 35.71% and 21.43% at the age of 2, 2.5 and 3 years, respectively. Serotype A, O and Asia-1 is circulated in Rajbari district and required trivalent vaccine for prevention and control of FMD in that area. SAARC J. Agric., 19(1): 201-210 (2021)
孟加拉国Rajbari地区最近暴发的杂交牛口蹄疫病毒的鉴定
口蹄疫是一种毁灭性的病毒性疾病,是孟加拉国的一种地方性疾病,造成巨大的经济损失。本研究的目的是在2018年1月至6月期间,分别通过rt - pcr和gsRT-PCR检测孟加拉国Rajbari地区杂交牛的口蹄疫疫情流行情况和FMDV血清型分子检测。从疑似临床口蹄疫的2 ~ 3岁杂交牛共采集了16份舌上皮样本。rt - pcr阳性14例。rt - pcr检测FMDV阳性率为87.50%。对uRT-PCR阳性标本进行gsRT-PCR血清分型。基于血清型的FMDV患病率分别为42.86%、100%、21.43%和21.43%,分别为O型、Asia-1型、A型和Asia-1和A混合感染。从年龄来看,2岁、2.5岁和3岁确诊口蹄疫暴发的患病率分别为42.86%、35.71%和21.43%。血清型A、O和亚洲-1型在Rajbari地区流行,该地区需要三价疫苗来预防和控制口蹄疫。南盟J.农业农业学报,19(1):201-210 (2021)
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