Immunohistochemical Evaluation of Gingival CD4+ lymphocytes in Response to Allograft Bone Powder (Ceno Bone) - Animal Study

ENVIRO Dental Journal Pub Date : 2021-06-30 DOI:10.12944/edj.03.01.05

Hadis Moradi, Ahmad Motaghi, A. Aminzadeh, Alireza Sadighi, Mohammad Hossien Shafazand

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Abstract

CD4 T lymphocytes play a central role in allergic reactions. Thus the present study aimed to, immunohistochemically, evaluate the presence of these lymphocytes in rabbit gingival tissues after the replacement of Cenobone. This experimental one way blinded study was performed on 20 gingival tissues gathered from disease-free rabbits with or without bone powder, respectively groups A and B. Immunohistochemical envision method was performed for mapping CD4 lymphocytes. The number and intensity of staining were compared between groups in 5 consequent HPF without overlap with the light microscope in connective tissue. Data were analyzed by Fisher exact test, Wilcoxon, and chi-square statistically in SPSS20 software. The number of CD4 T cells was higher in group A compared to group B.(P=0.02) Pattern of distribution in connective tissue did not show a difference between the two groups. (P=0.41). Results of the present study might confirm the role of CD4 T in an allergic reaction to bone powder material and suggest this cell as a useful factor for the prediction of allergic reactions in the first weeks of surgery. Further studies in this field are required.

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同种异体骨粉(Ceno Bone)对牙龈CD4+淋巴细胞反应的免疫组化评价-动物研究

CD4 T淋巴细胞在过敏反应中起核心作用。因此，本研究旨在通过免疫组织化学方法，评估兔牙龈组织中这些淋巴细胞的存在。本实验采用单盲法对20只无病兔的牙龈组织进行研究，分别为A组和b组。采用免疫组化想象法绘制CD4淋巴细胞图谱。比较结缔组织光镜下5组结缔组织无重叠HPF的染色数量和染色强度。采用SPSS20软件进行Fisher精确检验、Wilcoxon、卡方统计分析。A组CD4 T细胞数量明显高于b组(P=0.02)，结缔组织的分布模式在两组间无差异。(P = 0.41)。本研究的结果可能会证实CD4 T在骨粉物质过敏反应中的作用，并提示该细胞是预测手术头几周过敏反应的有用因素。这一领域需要进一步的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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ENVIRO Dental Journal

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