Beta-Glycosidase Activities of Lactobacillus spp. and Bifidobacterium spp. and The Effect of Different Physiological Conditions on Enzyme Activity

Q1 Engineering

Periodicals of Engineering and Natural Sciences Pub Date : 2023-01-27 DOI:10.28978/nesciences.1223571

Berat Cinar Acar, Z. Yüksekdağ

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引用次数: 1

Abstract

In this research, food (cheese, yoghurt) and animal (chicken) origin 39 Lactobacillus spp. and human origin (newborn faeces) three Bifidobacterium spp. were used. To designate the β-glycosidase enzyme and specific activities of the cultures, p-nitrophenyl-β-D glikopiranozit (p-NPG) was used as a substrate. The best specific activities between Lactobacilli cultures were observed at Lactobacillus rhamnosus BAZ78 (4.500 U/mg), L. rhamnosus SMP6-5 (2.670 U/mg), L. casei LB65 (3.000 U/mg) and L. casei LE4 (2.000 U/mg) strains. Bifidobacterium breve A28 (2.670 U/mg) and B. longum BASO15 (2.330 U/mg) strains belonging to the Bifidobacterium cultures had the highest specific activity capabilities. Optimization studies were performed to designate the impact of different pH, temperature, and carbon sources on the β-glucosidase enzyme of L. rhamnosus BAZ78 strain (β-Glu-BAZ78), which exhibits high specific activity. As optimum conditions, pH was detected as 7.5, the temperature as 30° C, and the carbon source as 2% glucose for the enzyme. Although the enzyme activity changed as the physiological conditions changed, the β-Glu-BAZ78 showed the highest specificity in the control groups.

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乳杆菌和双歧杆菌β -糖苷酶活性及不同生理条件对酶活性的影响

本研究采用食品(奶酪、酸奶)和动物(鸡)源性39株乳酸菌和人(新生儿粪便)源性3株双歧杆菌。以对硝基苯-β-D glikopiranozit (p-NPG)为底物，确定培养物的β-糖苷酶和特异性活性。鼠李糖乳杆菌BAZ78 (4.500 U/mg)、鼠李糖乳杆菌SMP6-5 (2.670 U/mg)、干酪乳杆菌LB65 (3.000 U/mg)和干酪乳杆菌LE4 (2.000 U/mg)培养物的比活性最高。双歧杆菌短双歧杆菌A28 (2.670 U/mg)和长双歧杆菌BASO15 (2.330 U/mg)菌株的比活能力最高。通过优化研究，确定了不同pH、温度和碳源对L. rhamnosus BAZ78菌株(β-Glu-BAZ78) β-葡萄糖苷酶具有高比活性的影响。最佳条件为pH为7.5，温度为30℃，碳源为2%葡萄糖。虽然酶活性随生理条件的变化而变化，但β-Glu-BAZ78在对照组中表现出最高的特异性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Periodicals of Engineering and Natural Sciences Engineering-Architecture

CiteScore

1.90

自引率

0.00%

发文量

140

审稿时长

7 weeks

期刊介绍： *Industrial Engineering: 1 . Ergonomics 2 . Manufacturing 3 . TQM/quality engineering, reliability/maintenance engineering 4 . Production Planning 5 . Facility location, layout, design, materials handling 6 . Education, case studies 7 . Inventory, logistics, transportation, supply chain management 8 . Management 9 . Project/operations management, scheduling 10 . Information systems for production and management 11 . Innovation, knowledge management, organizational learning *Mechanical Engineering: 1 . Energy 2 . Machine Design 3 . Engineering Materials 4 . Manufacturing 5 . Mechatronics & Robotics 6 . Transportation 7 . Fluid Mechanics 8 . Optical Engineering 9 . Nanotechnology 10 . Maintenance & Safety *Computer Science: 1 . Computational Intelligence 2 . Computer Graphics 3 . Data Mining 4 . Human-Centered Computing 5 . Internet and Web Computing 6 . Mobile and Cloud computing 7 . Software Engineering 8 . Online Social Networks *Electrical and electronics engineering 1 . Sensor, automation and instrumentation technology 2 . Telecommunications 3 . Power systems 4 . Electronics 5 . Nanotechnology *Architecture: 1 . Advanced digital applications in architecture practice and computation within Generative processes of design 2 . Computer science, biology and ecology connected with structural engineering 3 . Technology and sustainability in architecture *Bioengineering: 1 . Medical Sciences 2 . Biological and Biomedical Sciences 3 . Agriculture and Life Sciences 4 . Biology and neuroscience 5 . Biological Sciences (Botany, Forestry, Cell Biology, Marine Biology, Zoology) [...]