Use of Blood Mononuclear Cells Autologous Fraction for Treatment of Menstrual Disorders (Case Report)

Abstract

The aim of our work was to investigate the effectiveness of circulating SC in the blood mononuclear fraction to restore normal functioning of the human ovaries. AH270 patient complained to the clinic about an irregular menstrual cycle in the last 6 months (duration of the menstrual cycle from 38 to 60 days). On day 10 of the menstrual cycle, under general anesthesia, 2 ml of a solution containing a fraction of native nucleus-containing cells isolated from a pool of peripheral blood was administered transvaginally to the right and left ovaries. Microscopic examination of these cells showed that the obtained fraction contains polymorphic nucleus-containing cells of different sizes and high viability, which was 95-98%. After 2 months, the patient came for a follow-up examination, during which an ultrasound of the pelvic organs was performed, as well as laboratory diagnostics of blood tests for AMH and FSH levels. Were taken according to the results of ultrasound of the pelvic organs, the following changes were observed - in the right and left ovaries there were 2 antral follicles in each, up to 3 mm in diameter. The duration of the menstrual cycle after the introduction of stem cells has decreased to 30-35 days. Thus, it is shown that the pool of nuclear-containing peripheral blood cells, which contains circulating progenitors and stem cells, has a powerful regenerative effect with concentrated topical administration, which in this case led to a full functional recovery of the ovaries and normalization of the menstrual cycle.