Fluorescence microscopy with 3D resolution in the 100 nm range

Abstract

We propose a combination of 4Pi and Theta microscopies to improve the resolution of fluorescence microscopy by using six microscope objectives in a configuration we call Multiple Objective Microscopy (MOM). A resolution in the 100 nm range is obtained in the three dimensions using low numerical aperture, long working distance objectives. The obtained results not only show that high resolution is not restricted to high numerical aperture objectives, but also open the possibility of exploring large volumes with a very good resolution.