Screening, characterization and molecular insights of rhamnolipid biosurfactant produced by Pseudomonas aeruginosa BS1

Nisha S. Nayak, Dhruvi S. Joshi, Yesha S. Rathi
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Abstract

Biosurfactants are a group of heterogenous metabolites synthesized by a variety of microorganisms. They exhibit the properties of the surface tension reduction, emulsion stabilization, promote foaming, and specific activity at extreme temperatures, pH, and salinity. A bacterial strain was screened for its biosurfactant production in 250 ml MSM broth with crude oil as an inducer for 5 days. The screening activity performed by (i) drop collapse test, (ii) oil displacement test, (iii) emulsification index proved the presence of biosurfactant. TLC and FTIR analysis confirmed that the biosurfactant produced by the selected bacterial isolate is a rhamnolipid. The potential isolate was identified by 16S rRNA gene sequencing analysis and it was identified as Pseudomonas aeruginosa.
铜绿假单胞菌BS1产鼠李糖脂生物表面活性剂的筛选、表征及分子性质研究
生物表面活性剂是一组由多种微生物合成的异质代谢物。它们具有降低表面张力、稳定乳液、促进起泡和在极端温度、pH值和盐度下的比活性等特性。筛选了一株菌株,以原油为诱导剂,在250 ml MSM肉汤中培养5天。通过(i)滴落试验、(ii)驱油试验、(iii)乳化指数进行筛选,证明了生物表面活性剂的存在。TLC和FTIR分析证实所选细菌分离得到的生物表面活性剂为鼠李糖脂。经16S rRNA基因测序分析鉴定为铜绿假单胞菌(Pseudomonas aeruginosa)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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