Protective Effect of Ethosuximide against Glutamate-Induced Cytotoxicity and Oxidative Stress in C6 Glial Cells

Abstract

Purpose : The current study was intended to investigate the potential effect of ethosuximide on glutamate-induced cytotoxicity and oxidative stress in C6 glial cells. Material and Methods: In our study, the C6 cell line was used. To see how ethosuximide affected glutamate-induced cytotoxicity, four cell groups were created. The control group was without any treatment. Cells in the glutamate group were exposed to 10 mM glutamate for 24 h. Cells in the ethosuximide group were treated with various concentrations (5, 10, 20, and 40 μM) of ethosuximide for 24 h. Cells in the ethosuximide + glutamate group were pre-treated with various concentrations of ethosuximide for 1 h and then treated with glutamate for 24 h. The cell viability was measured using an XTT assay.Total oxidant status (TOS) and Total antioxidant status (TAS) in the cells were measured by commercial kits. Results: Ethosuximide at the concentrations of 10, 20, and 40 μM significantly increased the cell viability in C6 cells (p < 0.001). Ethosuximide at the concentration of 40 μM significantly decreased the TOS levels (p < 0.05) whereas didn’t alter the TAS levels (p > 0.05). Conclusion: Ethosuximide can cause neuroprotective effect on C6 cells. Additionally, one of the potential mechanisms involved in this effect is inhibition of oxidative stress. Alzheimer’s, Parkinson’s, Huntington’s