Root growth responses to mechanical impedance are regulated by a network of ROS, ethylene and auxin signalling in Arabidopsis

Amy G R Jacobsen, George Jervis, Jian Xu, J. Topping, K. Lindsey
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引用次数: 25

Abstract

The growth and development of root systems, essential for plant performance, is influenced by mechanical properties of the substrate in which the plants grow. Mechanical impedance, such as by compacted soil, can reduce root elongation and limit crop productivity. To understand better the mechanisms involved in plant root responses to mechanical impedance stress, we investigated changes in the root transcriptome and hormone signalling responses of Arabidopsis to artificial root barrier systems in vitro. We demonstrate that upon encountering a barrier, reduced Arabidopsis root growth and the characteristic ‘step-like’ growth pattern is due to a reduction in cell elongation associated with changes in signalling gene expression. Data from RNA-sequencing combined with reporter line and mutant studies identified essential roles for reactive oxygen species, ethylene and auxin signalling during the barrier response. We propose a model in which early responses to mechanical impedance include reactive oxygen signalling that is followed by integrated auxin and ethylene responses to mediate root growth changes. Inhibition of ethylene responses allows improved growth in response to root impedance, a result that may inform future crop breeding programmes.
拟南芥根系生长对机械阻抗的响应是由ROS、乙烯和生长素信号网络调控的
根系的生长和发育对植物的生长性能至关重要,它受到植物生长的基质力学特性的影响。机械阻抗,如压实土壤,可减少根伸长和限制作物产量。为了更好地了解植物根系对机械阻抗胁迫的响应机制,我们在体外研究了拟南芥对人工根屏障系统的根转录组和激素信号响应的变化。我们证明,在遇到屏障时,拟南芥根系生长减少和典型的“阶梯状”生长模式是由于与信号基因表达变化相关的细胞伸长减少。rna测序结合报告系和突变体研究的数据确定了活性氧、乙烯和生长素信号在屏障反应中的重要作用。我们提出了一个模型,在该模型中,对机械阻抗的早期响应包括活性氧信号,随后是生长素和乙烯的综合响应,以介导根的生长变化。抑制乙烯反应可以改善对根阻抗的生长反应,这一结果可能为未来的作物育种计划提供信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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