Common Microorganisms Present in Diabetic Foot Infection and It’s Spectrum of Antibiotic Sensitivity

Q4 Medicine

Medicine Today Pub Date : 2022-04-24 DOI:10.3329/medtoday.v34i1.58680

Md Ziaur Rahman, Mir Jalal Uddin, A. Mollah, Himika Hasan khan, Akm Ziaul Kabir

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Abstract

Introduction: Diabetic patients have foot problems secondary to neuropathy, micro-vascular changes and impaired resistance to infection. They are at increased risk of infection and ulceration. Diabetic ulcers are frequently involved in both aerobe and anaerobe microorganisms. It is important to culture the specimen on several different media. The Gram stain can provide valuable information regarding the range of organisms under consideration. The aim of this study is to identify the sensitive antibiotics to the specific aerobic bacteria, to prevent the random misuse of broad spectrum antibiotics and make awareness about diabetic foot care. Materials and Methods: This study was a retrospective analysis and was carried out in general surgery and diabetic wards at North Bengal Medical College Hospital (NBMCH), Sirajganj, Bangladesh from August, 2019 to July, 2020. To identify common aerobic microorganism and spectrum of antibiotic sensitivity from 54 cases of diabetic foot ulcers, wound swabs taken and cultured using standard aerobic microbiological techniques. Antibiotic sensitivity testing to different antimicrobial agents were carried out using the disc diffusion method. Result: Among 54 aerobic bacterial isolates, Gram negative bacteria 41(75.9%) and Gram positive bacteria 13(24.1%). Escherichia Coli 28(51.9%), Proteus spp 09(16.7%), Pseudomonas 03(5.6%), Klebsiella spp 01(1.9%) were isolated Gram negative bacteria. Staphylococcus aureus 13(24.1%) was only Gram positive bacteria. Gram negative bacterial isolates were sensitive to Imipenem, while Vancomycin showed good activity against Gram positive bacteria. Conclusion: The antibiogram results of this study suggest that bacteria remain sensitive to a limited number of used agents, while found resistant to a number of widely used agents. Imipenem was most effective against Gram negative bacilli and also effective against Gram positive cocci. Medicine Today 2022 Vol.34(1): 65-69

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糖尿病足感染中的常见微生物及其抗生素敏感性谱

导读:糖尿病患者有继发于神经病变、微血管改变和感染抵抗力受损的足部问题。他们感染和溃烂的风险增加。糖尿病溃疡常与需氧微生物和厌氧微生物有关。在几种不同的培养基上培养标本是很重要的。革兰氏染色可以提供有关所考虑的生物体范围的有价值的信息。本研究旨在确定对特定需氧菌敏感的抗生素，防止广谱抗生素的随机滥用，提高糖尿病足护理意识。材料与方法:本研究为回顾性分析，于2019年8月至2020年7月在孟加拉国Sirajganj北孟加拉医学院医院(NBMCH)的普通外科和糖尿病病房进行。采用标准好氧微生物学技术对54例糖尿病足溃疡创面拭子进行培养，鉴定常见好氧微生物及其抗生素敏感性谱。采用纸片扩散法对不同抗菌药物进行了药敏试验。结果:54株需氧菌中革兰阴性菌41株(75.9%)，革兰阳性菌13株(24.1%)。革兰氏阴性菌分别为大肠杆菌28(51.9%)、变形杆菌09(16.7%)、假单胞菌03(5.6%)、克雷伯菌01(1.9%)。金黄色葡萄球菌13(24.1%)仅为革兰氏阳性菌。革兰氏阴性菌对亚胺培南敏感，而万古霉素对革兰氏阳性菌有较好的抑制作用。结论:本研究的抗生素谱结果表明，细菌对有限数量的使用药物保持敏感，而对一些广泛使用的药物发现耐药。亚胺培南对革兰氏阴性杆菌最有效，对革兰氏阳性球菌也有效。今日医学2022 Vol.34(1): 65-69

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Medicine Today Medicine-Medicine (all)

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