Isolation and Partial Characterisation of Bacteriocin Produced By Lactobacillus Fermentum

Abstract

The present study was confined to the velar estuary in Cuddalore District, Tamil Nadu.Water samples were collected to isolate the bacteria. The collected samples were plated (MRS agar), incubated and the Bacterial colonies were identified. Effect of different ecological parameters such as pH (6-11), temperature (30-60oC), salinity (0.5-3%), substrates (carbon &nitrogen source) on the growth of bacteria was also determined. Maximum bacterial growth was observed in pH 9, 35oC temperature, 2% salinity, 1% glucose as carbon source and 0.5% sodium nitrate as nitrogen source after 24hrs of growth in liquid medium. The potential strain was selected by the antimicrobial well assay method. Lactobacillus fermentum (Bacteriocin) contains inhibitory activity against histamine producers 10 strains) . Antimicrobial activity was tested against 10 human pathogens. The protein bands were observed using SDS-PAGE and their molecular weight ranging from 78KDa.