Intervention effect of lecithin on cell membrane injury of African green monkey kidney exposed to sodium arsenite in vitro

Q3 Medicine

中华地方病学杂志 Pub Date : 2011-07-20 DOI:10.3760/CMA.J.ISSN.1000-4955.2011.04.013

Tingxin Wang, Ya-lou Zhang, Ji-Wen Liu, Shengling Wang

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引用次数: 0

Abstract

Objective To observe the lecithin's effect on membrane of African green monkey kidney cells (Vero) exposed to sodium arsenite(NaAsO2). Methods Vero cells cultured in vitro were divided into 4 groups:control group (saline), model group (2.20 mg/L NaAsO2), high eoncentration of lecithin and arsenic group (53.33mg/L lecithin + 2.20 mg/L NaAsO2), low eoncentration of lecithin and arsenic group( 13.32 mg/L lecithin + 2.20 mg/L NaAsO2), 6 bottles of cells in each group, medium was changed every 2 days, cultured for 120 h. Na+ ,K+-ATPase activities of membrane were measured by spectrophotometry, and membrane phospholipids composition including phosphatidylserine (PS), phosphatidylethano-lamine (PE), phosphatidylcholine (PC) and sphingmyelin (SM) were measured by high performance liquid chromatography (HPLC). Results The Na~, K+-ATPase activities of membrane of control group, model group, high concentration of lecithin and arsenic group, low concentration of lecithin and arsenic group were (0.962 ± 0.081) × 106, (0.544 ± 0.037) × 106, (0.647 ± 0.043) x 106, (0.550±Compared with control group, the Na+ ,K+-ATPase activities of other 3 groups were significantly reduced (all P 0.05). Compared with control group[(0.087 ± 0.003), (0.127 ± 0.053), (0.588 ± 0.105),(0.071 ± 0.029)g/L], PS, PE, PC, SM levels in model group[(0.051 ± 0.018), (0.073 + 0.030), (0.240 ±0.038), (0.047 ± 0.121 )g/L] were significantly lower(all P 0.05), but SM[(0.057 ± 0.004)g/L] significantly decreased(P 0.05]. Compared with model group,the levels of PS, PE, PC, SM in high concentration of lecithin and arsenic group were significantly higher(all P 0.05), and PC was significantly higher(P < 0.05). Conclusions High concentration lecithin has certain protective effect on Vero cell membrane exposured to sodium arsenite. Key words: Arsenites ; Phosphatidylcholines; Cell membrane; Na+-K+-exchanging ATPase

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卵磷脂对体外亚砷酸钠作用下非洲绿猴肾细胞膜损伤的干预作用

目的观察卵磷脂对亚砷酸钠(NaAsO2)作用下非洲绿猴肾细胞(Vero)膜的影响。方法将体外培养的Vero细胞分为4组:对照组(生理盐水)、模型组(2.20 mg/L NaAsO2)、高浓度卵磷脂和砷组(53.33mg/L卵磷脂+ 2.20 mg/L NaAsO2)、低浓度卵磷脂和砷组(13.32 mg/L卵磷脂+ 2.20 mg/L NaAsO2)，每组6瓶，每2 d换一次培养基，培养120 h，分光光度法测定细胞膜Na+、K+- atp酶活性。采用高效液相色谱法测定膜磷脂组成，包括磷脂酰丝氨酸(PS)、磷脂酰乙醇胺(PE)、磷脂酰胆碱(PC)和鞘髓磷脂(SM)。结果对照组、模型组、高浓度卵磷脂和砷组、低浓度卵磷脂和砷组细胞膜Na~、K+- atp酶活性分别为(0.962±0.081)× 106、(0.544±0.037)× 106、(0.647±0.043)× 106、(0.550±)，与对照组比较，其他3组细胞膜Na+、K+- atp酶活性均显著降低(均P 0.05)。与对照组[(0.087±0.003)、(0.127±0.053)、(0.588±0.105)、(0.071±0.029)g/L]相比，模型组PS、PE、PC、SM水平[(0.051±0.018)、(0.073 + 0.030)、(0.240±0.038)、(0.047±0.121)g/L]显著降低(P 0.05)， SM[(0.057±0.004)g/L]显著降低(P 0.05)。与模型组相比，高浓度卵磷脂和砷组PS、PE、PC、SM水平显著升高(均P 0.05)， PC水平显著升高(P < 0.05)。结论高浓度卵磷脂对亚砷酸钠暴露的Vero细胞膜有一定的保护作用。关键词:亚砷酸盐;磷脂酰胆碱;细胞膜;Na + - k +交换腺苷三磷酸酶

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来源期刊

中华地方病学杂志我国对人类健康危害特别严重的地方性疾病：克山病、大骨节病、碘缺乏病、地方性氟中毒、地方性砷中毒、鼠疫、布鲁氏菌病、寄生虫、新冠肺炎等疾病，同时还报道多发性自然疫源性疾病。

CiteScore

1.60

自引率

0.00%

发文量

8714

期刊介绍： The Chinese Journal of Endemiology covers predominantly endemic diseases threatening health of the people in the areas affected by the diseases including Keshan disease, Kaschin-Beck Disease, iodine deficiency disorders, endemic fluorosis, endemic arsenism, plague, epidemic hemorrhagic fever, brucellosis, parasite diseases and the diseases related to local natural and socioeconomic conditions; and reports researches in the basic science, etiology, epidemiology, clinical practice, control as well as multidisciplinary studies on the diseases.