The phenolic constituents and antimicrobial activity of Xanthium spinosum (asteraceae) extracts

IF 0.9 4区生物学 Q4 PLANT SCIENCES

Botanica Serbica Pub Date : 2023-01-01 DOI:10.2298/botserb2301093m

Milica Miletić, Marija Ivanov, A. Topalović, M. Gavrilović, U. Gašić, P. Janaćković

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Abstract

Xanthium spinosum is a cosmopolitan annual herb used in traditional medicine worldwide. Although known from ethnobotanical studies, the species is scarcely investigated from the aspects of phytochemistry and biological activity. Therefore, the phenolic composition and biological activity of X. spinosum were examined. Plant specialised metabolites (phenolics) extracted from the roots, leaves and fruits with dichloromethane:methanol (1:1) were analysed by liquid chromatography mass spectrometry (LC-MS). In total 10 phenolic compounds were identified and quantified. Six compounds were common to all the extracts. Chlorogenic acid was the most abundant constituent in all the extracts (4.262 mg/g in the fruit extract, 0.820 mg/g in the leaf extract, and 0.540 mg/g in the root extract). The biological activity (antimicrobial and antibiofilm) of the extracts was tested against 12 microfungi and 12 bacterial strains by the microdilution method. All the extracts exhibited moderate antimicrobial and antibiofilm activity and inhibited the growth of most of the examined microorganisms. The obtained results indicate the potential role of the tested extracts in pharmacy and medicine.

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苍耳草提取物的酚类成分及其抑菌活性

苍耳是一种世界性的一年生草本植物，在世界各地的传统医学中都有使用。虽然从民族植物学研究中已知，但很少从植物化学和生物活性方面对该物种进行调查。为此，对棘草的酚类成分和生物活性进行了研究。用二氯甲烷:甲醇(1:1)提取根、叶和果实中的植物专用代谢物(酚类物质)，采用液相色谱-质谱(LC-MS)分析。共鉴定并定量了10种酚类化合物。6种化合物是所有提取物共有的。绿原酸含量最高(果实4.262 mg/g，叶0.820 mg/g，根0.540 mg/g)。采用微量稀释法测定了提取物对12种微真菌和12种细菌的生物活性(抗菌活性和抗生物膜活性)。所有提取物都表现出适度的抗菌和抗生物膜活性，并抑制了大多数被检测微生物的生长。结果表明，所测提取物在药学和医学上具有潜在的作用。

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来源期刊

Botanica Serbica Agricultural and Biological Sciences-Plant Science

CiteScore

1.40

自引率

12.50%

发文量

审稿时长

34 weeks

期刊介绍： Botanica Serbica publishes original research papers on all aspects of plant, fungal and microbial biology research including the disciplines of microbiology, mycology, lichenology, bryology, flora, vegetation, biogeography, systematics, taxonomy, plant biotechnology, plant cell biology, plant ecology, environmental plant biology, forestry, genomics, horticulture, limnology, metabolomics, molecular biology, proteomics, virology, plant conservation and protection, and wildlife and ecosystem management.