Advances in in vitro culture of Blastocystis hominis

Abstract

Blastocystis hominis , a protozoan parasite commonly found in the gut of humans and animals, is closely related to a variety of gastrointestinal diseases, such as abdominal pain, diarrhea and intestinal mucosal damage. B. hominis is distributed all over the world. The in vitro culture of B. hominis is of great significance for the clinical diagnosis of B. hominis infection and the establishment of B. hominis animal model, the study of genotype and pathogenic mechanism and so on. The selection of efficient and economical culture medium lays the foundation for the follow-up study of B. hominis life cycle, morphological classification, infection immunity and so on. The state, type, serum concentration of culture medium and culture conditions are important factors affecting the culture of B. hominis in vitro . This review summarizes the research progress of B. hominis culture in vitro and the culture conditions suitable for rapid growth and propagation of B. hominis under different physical conditions, expounds the differences between different media and the factors affecting the culture of B. hominis in vitro , in order to provide reference for clinical and scientific researchers to select suitable culture medium and culture conditions. 摘要： 人芽囊原虫( Blastocystis hominis )呈世界性分布,是一种常见的人和动物肠道内寄生原虫,与多种胃肠道疾病 (如腹痛、腹泻、肠黏膜损伤)密切相关。体外培养人芽囊原虫对于临床诊断人芽囊原虫感染、建立人芽囊原虫动物模 型、进行基因型和致病机制等研究具有重要意义;选择髙效经济的培养基为人芽囊原虫的生活史、形态分类、感染免疫 等后续研究奠定基础。培养基的状态、种类、血清浓度和培养条件等是影响人芽囊原虫体外培养的重要因素。本综述 总结了人芽囊原虫体外培养的研究进展及不同物理状态下适合人芽囊原虫快速生长繁殖的培养条件,阐述了不同培 养基之间的差异及影响人芽囊原虫体外培养的因素,旨在为临床和科研工作者选择合适的培养基及培养条件提供 参考。