Genetic characterisation of centuries-old oak and linden trees using SSR markers

Q3 Agricultural and Biological Sciences

Folia Forestalia Polonica, Series A Pub Date : 2022-03-01 DOI:10.2478/ffp-2022-0006

S. Bilous, L. Prysiazhniuk, S. Chernii, Serghii Melnyk, Yurii N. Marchuk, A. Likhanov

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引用次数: 1

Abstract

Abstract The main goal of this study was to identify the unique gene pool of old and historically valuable Quercus robur L. and Tilia cordata L. to be able to characterise their genetic diversity in order to determine the polymorphism by expressed sequence tag-single sequence repeat (EST-SSR) markers and identify the most valuable specimens. Morphological description, molecular genetic analysis, and statistical analysis were used in studies. The genetic distances between old-value trees of different Quercus L. and Tilia L. were determined based on EST-SSR markers and morphological characteristics. Using polymerase chain reaction (PCR), alleles of the expected size were obtained. It was determined that four to eight alleles were obtained by seven SSR markers in the studied Q. robur L. samples. According to the calculated value of the locus polymorphism index (polymorphism information content [PIC]), the most polymorphic was the marker SSRQrZAG 65; the PIC was 0.84. The lowest value of PIC was observed in the marker SSRQrZAG 11; the PIC was 0.69. Intragenetic polymorphism was detected for all studied markers. Among the studied samples of linden, two to five alleles were identified. It was found that the highest value of PIC was obtained for the marker Ts920 – 0.72. The least polymorphic was the marker Ts927 (PIC was 0.28), which is not only due to the small number of alleles, but also their uneven distribution in the sample. Intragenetic polymorphism was detected in four of the six markers analysed for T. cordata L. In this study, polymorphism was detected in all studied samples of Q. robur L. and T. cordata L., which allows to assess their genetic diversity based on the distribution of alleles.

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利用SSR标记分析百年橡树和椴树的遗传特征

摘要本研究的主要目的是鉴定具有历史价值的古栎(Quercus robur L.)和天麻(Tilia cordata L.)的独特基因库，以表征其遗传多样性，并利用EST-SSR(表达序列标记-单序列重复)标记确定其多态性，从而筛选出最有价值的标本。形态学描述、分子遗传分析和统计学分析均被用于研究。利用EST-SSR标记和形态特征，确定了不同栎、椴老树间的遗传距离。利用聚合酶链反应(PCR)获得了预期大小的等位基因。结果表明，7个SSR标记可获得4 ~ 8个等位基因。根据位点多态性指数(多态性信息含量[PIC])的计算值，多态性最多的是标记SSRQrZAG 65;PIC为0.84。标记SSRQrZAG 11的PIC值最低;PIC为0.69。所有研究标记均检测到遗传内多态性。在所研究的椴树样品中，鉴定出2 ~ 5个等位基因。结果表明，标记Ts920 - 0.72的PIC值最高。多态性最小的是标记Ts927 (PIC为0.28)，这不仅是由于等位基因数量少，而且它们在样本中的分布不均匀。结果表明，6个标记中有4个标记存在遗传内多态性，所有样本均存在遗传内多态性，可根据等位基因的分布来评估其遗传多样性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Folia Forestalia Polonica, Series A Agricultural and Biological Sciences-Forestry

CiteScore

1.30

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： FOLIA FORESTALIA POLONICA, SERIES A – FORESTRY is a forest science magazine addressed to scientists, administrators and policy-makers in forestry, agroforestry, ecology, environment and resource management. The language of publication is English and papers from any region of the world are welcome.