Light and Scanning Electron Microscopy of Some Species of Genus Solanum (Solanaceae) With Special Referance to the Importance of Trichomes

Abstract

The trichomes are taxonomically very important because of their use in plant classification with other morphological characters. This study provides information about trichomes of six species add name of species here of genus Solanum. This study will also helpful to produce more information to develop modern nomenclature for trichomes. The plant material was taken from Sindh University Herbarium as well as new plant substance was gathered from the field for the study. During the trichomal analysis the plant components were observed under stereo zoom microscope. Provisional and long-term slides were produced. For the scanning electron microscopic observation, the plant material was treated with ethanol alcohol for 20 minutes, ca 1mm2 piece of specimens were fitted on the stubs made of metal with double-sided cellophane tape and also covered with C-30-50 nm gold by Jeol JFC- 1500 Ion sputter machine. After coating samples were observed as well as pictures were captured on different magnifications by enhancing voltage between 5-15 KV by Scanning Electron Microscope (SEM) Jeol JSM-T200 & Jeol JSM-T6380 at Biological Research Centre and Central Research Laboratory Karachi, University of Karachi.