Quantitative estimation of urinary ortho-cresol by high pressure liquid chromatography for biomonitoring of toluene exposed population

Environmental Disease Pub Date : 2020-07-01 DOI:10.4103/ed.ed_23_20

A. Yadav, A. Chakrabarti, Geoffrey Nengzapum

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Abstract

Aim: Quantification of urinary ortho-cresol (OC) by high pressure liquid chromatography coupled with photodiode array detector. Materials and Methods: Includes acid hydrolysis of urine, liquid–liquid extraction, and chromatography quantification of extracted OC in urine. Results: Limit of detection, limit of quantification, and coefficient of linearity (R2) were 0.18 μg/ml, 0.62 μg/ml, and 0.9998, respectively. Recovery % of method ranged from 92%, 97%, and 100%. For intraday and interday precision coefficient of variation was 0.41%, 0.64%, and 0.89%, 0.86% for urine samples spiked with OC standards final concentration of 0.25 μg/ml and 0.7 μg/ml, respectively. Results (mean ± standard deviation) of exposed and unexposed real urine samples analyzed for OC with this method were 0.92 ± 0.76 and 0.40 ± 0.20 μg/ml, respectively. Statistical analysis of results showed significant (P ≤ 0.001) difference between urinary OC among exposed and unexposed subjects. Conclusion: The present work describes precise, easy, and less time consuming method for estimation of OC in urine of population exposed to toluene. It can be used as a promising tool for biomonitoring of population exposed to toluene.

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用高压液相色谱法定量测定尿中邻甲酚对甲苯暴露人群的生物监测

目的:采用高压液相色谱-光电二极管阵列检测器定量测定尿中邻甲酚(OC)。材料和方法:包括尿液的酸水解，液-液萃取，以及尿液中提取OC的色谱定量。结果:检测限为0.18 μg/ml，定量限为0.62 μg/ml，线性系数(R2)为0.9998。方法回收率为92%、97%、100%。当OC标准终浓度为0.25 μg/ml和0.7 μg/ml时，日内和日间的精密度变异系数分别为0.41%、0.64%和0.89%、0.86%。用该方法对暴露和未暴露的真实尿样进行检测，结果(平均值±标准差)分别为0.92±0.76和0.40±0.20 μg/ml。统计分析结果显示，暴露者与未暴露者尿OC差异有统计学意义(P≤0.001)。结论:本工作描述了一种准确、简便、省时的甲苯暴露人群尿液OC测定方法。它可作为一种有前景的甲苯暴露人群生物监测工具。

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Environmental Disease

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