Hydrodynamically Activated Sawdust of Common Pine Pinus sylvestris L.-substrate for Cultivation of the Strain Gl4–16A Ganoderma lucidum

Abstract

The results of a study on the use of sawdust of common pine Pinus sylvestris L. with their preliminary activation in a cavitation water-impact dispersant for 25 minutes as a substrate for cultivating the Gl4–16A strain of Ganoderma lucidum are presented. It was found that the Gl4–16A strain of Ganoderma lucidum colonizes the substrate more efficiently with the introduction of (NH4)2SO4 and Na2HPO4 salts into its composition. At the same time, the average growth rate of basidiomycete on the substrate without the introduction of salts was 1.39±0.53 mm/day, and on the substrate with nitrogen salts – 3.22±0.48 mm/day, and complete fouling of the substrate occurred on day 13. It was found that during the cultivation of the Gl4–16A strain of Ganoderma lucidum on a substrate with nitrogen salts, the content of extractive substances in it increases from 4.37 % to 6.32 %, where more than 65 % is accounted for by substances extracted with water. There is a decrease in the content of difficult-to-hydrolyze polysaccharides by 13.3 %, and an increase in the non-hydrolyzable part by 2.4 %, which is 6.8 % per organic mass. The content of easily hydrolyzable polysaccharides (LGA) decreases by 2.4 %, but this change is not significant in terms of organic mass and the share of LGA both before and after bioconversion is about 15.7 %. The obtained data can expand the field of use of sawdust, which is a promising direction, both in the chemical and pharmaceutical industries