Bioconversions of olive oil mill wastewaters blends

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Abstract

The ability of the anaerobic mixed bacterial culture from an upflow anaerobic sludge blanket (UASB) bioreactor to convert blends of olive mill wastewaters (OMWs) with synthetic glucose medium or molasses into ethanol simultaneously with organic acids (OAs) was studied in the present work. All fermentations were conducted by free cells under non-aerated conditions at 37 οC and the effect of OMWs concentration on ethanol and OAs production was investigated. The highest amount of bioethanol (14.7 g L-1) was produced during fermentation of 45 % v/v OMWs mixed with synthetic glucose medium (5 % w/v) in only 28 hours. In mixtures of 30 % v/v OMWs and molasses solution (3 oBe) 13.4 g L-1 of bioethanol were produced. Also, 16.2 g L-1 of succinic acid were produced, when 65 % v/v OMWs mixed with synthetic glucose medium. Moreover, totally 11.6 g L-1 of varied OAs (succinic, malic, butyric and acetic) observed in mixtures of 35 % OMWs with molasses. Finally, it has been shown that the 14C-labelled glucose uptake rate (GUR) by biomass was strongly correlated to fermentation rate.
橄榄油厂废水混合物的生物转化
研究了上流式厌氧污泥毯(UASB)生物反应器厌氧混合细菌培养将橄榄厂废水(OMWs)与合成葡萄糖培养基或糖蜜混合转化为乙醇和有机酸(OAs)的能力。所有发酵均由游离细胞在37 o o c无曝气条件下进行,并研究了OMWs浓度对乙醇和OAs产量的影响。45% v/v的OMWs与5% w/v的合成葡萄糖培养基在28小时内发酵产生最高的生物乙醇(14.7 g L-1)。在30% v/v的OMWs和糖蜜溶液(3 oBe)的混合物中,生产13.4 g L-1的生物乙醇。当65% v/v的OMWs与合成葡萄糖培养基混合时,琥珀酸的产量为16.2 g L-1。此外,在35%的OMWs与糖蜜的混合物中,共观察到11.6 g L-1的各种oa(琥珀酸、苹果酸、丁酸和乙酸)。最后,研究表明,生物质的14c标记葡萄糖摄取速率(GUR)与发酵速率密切相关。
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